Characterization of Human Mast Cells Developed in Vitro from Fetal Liver Cells Cocultured with Murine 3T3 Fibroblasts

Overview

Journal Immunology

Specialty Allergy & Immunology

Date 1992 Sep 1

PMID 1398760

Citations 9

Authors

A A Irani

S S Craig

G Nilsson

T Ishizaka

L B Schwartz

Affiliations

Soon will be listed here.

Abstract

Cocultures of dispersed human fetal liver cells with murine Swiss 3T3 fibroblasts resulted in the development of human mast cells after 1 to 4 weeks of culture. Mast cells were detected by immunohistochemistry using a murine monoclonal anti-tryptase antibody, before metachromasia appeared with toluidine blue. When subjected to double immunohistochemistry using murine monoclonal anti-chymase and anti-tryptase antibodies, 94% +/- 10% (SD) of the mast cells seen at day 30 of culture were of the MCT type. These results contrast with those obtained with human mast cells derived from cord blood mononuclear cells cocultured with murine 3T3 fibroblasts which are comprised of substantially greater numbers of MCTC cells, averaging 48% +/- 31% (SD) at day 30 of culture. Mast cells developed in vitro from fetal liver cells or cord blood mononuclear cells contained similar amounts (+/- SD) of histamine (0.9 +/- 0.5 pg/cell and 1.1 +/- 1 pg/cell, respectively) and tryptase (1.7 +/- 0.4 pg/cell and 1.9 +/- 1.2 pg/cell, respectively) on day 30 of culture. Fetal-liver-derived mast cells from a 30-day-old culture were identified by immunoelectron microscopy using gold-labelled antitryptase antibody. Typically, these mast cells appeared immature as they had large nuclear to cytoplasmic ratio and a small number of ill-formed cytoplasmic granules. For both fetal-liver- and cord-blood-derived mast cells, there was no evidence of conversion of the MCT type into the MCTC type provided by this study. These results suggest that commitment to develop as an MCT or MCTC type of mast cell may have occurred in mast cell precursors present in fetal liver and cord blood mononuclear cells, prior to granulation.

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References

Woodbury R, NEURATH H . Purification of an atypical mast cell protease and its levels in developing rats. Biochemistry. 1978; 17(20):4298-304. DOI: 10.1021/bi00613a029. View

Irani A, Goldstein S, Wintroub B, Bradford T, Schwartz L . Human mast cell carboxypeptidase. Selective localization to MCTC cells. J Immunol. 1991; 147(1):247-53. View

Schechter N, Irani A, Sprows J, Abernethy J, Wintroub B, Schwartz L . Identification of a cathepsin G-like proteinase in the MCTC type of human mast cell. J Immunol. 1990; 145(8):2652-61. View

Okamoto H, Nakano K . Regulation of interleukin-1 synthesis by histamine produced by mouse peritoneal macrophages per se. Immunology. 1990; 69(1):162-5. PMC: 1385737. View

Seldin D, Caulfield J, Hein A, Osathanondh R, Nabel G, Schlossman S . Biochemical and phenotypic characterization of human basophilic cells derived from dispersed fetal liver with murine T cell factors. J Immunol. 1986; 136(6):2222-30. View

Craig S, Schechter N, Schwartz L . Ultrastructural analysis of human T and TC mast cells identified by immunoelectron microscopy. Lab Invest. 1988; 58(6):682-91. View

Irani A, Schwartz L . Mast cell heterogeneity. Clin Exp Allergy. 1989; 19(2):143-55. DOI: 10.1111/j.1365-2222.1989.tb02357.x. View

Craig S, Schechter N, Schwartz L . Ultrastructural analysis of maturing human T and TC mast cells in situ. Lab Invest. 1989; 60(1):147-57. View

Irani A, Bradford T, Kepley C, Schechter N, Schwartz L . Detection of MCT and MCTC types of human mast cells by immunohistochemistry using new monoclonal anti-tryptase and anti-chymase antibodies. J Histochem Cytochem. 1989; 37(10):1509-15. DOI: 10.1177/37.10.2674273. View

10.

Irani A, Craig S, Deblois G, Elson C, Schechter N, Schwartz L . Deficiency of the tryptase-positive, chymase-negative mast cell type in gastrointestinal mucosa of patients with defective T lymphocyte function. J Immunol. 1987; 138(12):4381-6. View

11.

Castells M, Irani A, Schwartz L . Evaluation of human peripheral blood leukocytes for mast cell tryptase. J Immunol. 1987; 138(7):2184-9. View

12.

Huang E, Nocka K, Beier D, Chu T, Buck J, Lahm H . The hematopoietic growth factor KL is encoded by the Sl locus and is the ligand of the c-kit receptor, the gene product of the W locus. Cell. 1990; 63(1):225-33. DOI: 10.1016/0092-8674(90)90303-v. View

13.

Braganza V, SIMMONS W . Tryptase from rat skin: purification and properties. Biochemistry. 1991; 30(20):4997-5007. DOI: 10.1021/bi00234a023. View

14.

Irani A, Schwartz L . Neutral proteases as indicators of human mast cell heterogeneity. Monogr Allergy. 1990; 27:146-62. View

15.

Martin F, Suggs S, Langley K, Lu H, Ting J, Okino K . Primary structure and functional expression of rat and human stem cell factor DNAs. Cell. 1990; 63(1):203-11. DOI: 10.1016/0092-8674(90)90301-t. View

16.

Craig S, Schwartz L . Human MCTC type of mast cell granule: the uncommon occurrence of discrete scrolls associated with focal absence of chymase. Lab Invest. 1990; 63(4):581-5. View

17.

Ishizaka T, Conrad D, Huff T, Metcalfe D, Stevens R, Lewis R . Unique features of human basophilic granulocytes developed in in vitro culture. Int Arch Allergy Appl Immunol. 1985; 77(1-2):137-43. DOI: 10.1159/000233768. View

18.

Woldemussie E, AIKEN D, BEAVEN M . Evidence for rapid histamine turnover and loss of histamine from immature rat mast cells. Biochem Pharmacol. 1986; 35(6):903-9. DOI: 10.1016/0006-2952(86)90075-4. View

19.

Levi-Schaffer F, Austen K, Caulfield J, Hein A, Gravallese P, Stevens R . Co-culture of human lung-derived mast cells with mouse 3T3 fibroblasts: morphology and IgE-mediated release of histamine, prostaglandin D2, and leukotrienes. J Immunol. 1987; 139(2):494-500. View

20.

Chabot B, Stephenson D, Chapman V, Besmer P, Bernstein A . The proto-oncogene c-kit encoding a transmembrane tyrosine kinase receptor maps to the mouse W locus. Nature. 1988; 335(6185):88-9. DOI: 10.1038/335088a0. View