Development of a Fixed Bed Bioreactor for the Expansion of Human Hematopoietic Progenitor Cells

Overview

Journal Cytotechnology

Specialties Biotechnology
Genetics

Date 2008 Nov 13

PMID 19003372

Citations 9

Authors

P Meissner

B Schroder

C Herfurth

M Biselli

Affiliations

Soon will be listed here.

Abstract

The ex vivo expansion of hematopoietic progenitor cells is of great interest for a variety of clinical applications, e.g. bone marrow transplantation or gene therapy. Therefore it is of general interest to develop a culture system, able to mimic the in vivo hematopoesis, which is a prerequisite for long-term hematopoietic culture. Our approach was to modify a continuously perfused bioreactor for cultivation and expansion of human hematopoietic stem cells. Therefore we immobilized stromal cells (human primary stromal cells or the murine cell line M2-10B4) in porous glass carriers in a fixed bed reactor and cocultivated human hematopoietic progenitor cells for several weeks. After inoculation of mononuclear cells derived from umbilical cord blood or peripheral blood stem cells both adherent and non adherent cells were harvested and analyzed by flow cytometry and short-term colony assays. During cultivation there was a permanent production of progenitor cells and mature blood cells derived from the immobilized cells in the carriers. We could demonstrate the immobilization of hematopoietic progenitor cells of the myeloid system detectable in short-term colony assays. Additionally we could observe the expansion of very early progenitor cells (CFU-GEMM) up to 4.2-fold and later progenitor cells (CFU-GM and BFU-E) up to 7-fold and 1.8-fold, respectively.

Citing Articles

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Scale-Up Technologies for the Manufacture of Adherent Cells.

Bellani C, Ajeian J, Duffy L, Miotto M, Groenewegen L, Connon C Front Nutr. 2020; 7:575146.

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Synergistic Integration of Mesenchymal Stem Cells and Hydrostatic Pressure in the Expansion and Maintenance of Human Hematopoietic/Progenitor Cells.

Kang Y, Jeong J, Lee T, Lee H, Shin J Stem Cells Int. 2018; 2018:4527929.

PMID: 29681947 PMC: 5848107. DOI: 10.1155/2018/4527929.

Stromalized microreactor supports murine hematopoietic progenitor enrichment.

Khong D, Li M, Singleton A, Chin L, Parekkadan B Biomed Microdevices. 2018; 20(1):13.

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