Assessment of Antimicrobial and Wound Healing Effects of Brevinin-2Ta Against the Bacterium in Dermally-wounded Rats

Overview

Journal Oncotarget

Specialty Oncology

Date 2018 Jan 18

PMID 29340060

Citations 19

Authors

Siqin Liu

Qilin Long

Yang Xu

Jun Wang

Zhongwei Xu

Lei Wang

Mei Zhou

Yuxin Wu

Tianbao Chen

Chris Shaw

Affiliations

Soon will be listed here.

Abstract

Antimicrobial peptides (AMPs) are regarded as promising alternatives for antibiotics due to their inherent capacity to prevent microbial drug resistance. Amphibians are rich source of bioactive molecules, which provide numerous AMPs with various structures as drug candidates. Here, we isolated and identified a novel AMP Brevinin-2Ta (B-2Ta) from the skin secretion of the European frog, kl. . studies revealed that it showed broad antimicrobial activities against , and with low cytotoxicity to erythrocytes. Furthermore, we examined the anti-inflammation effect by using -infected Sprague-Dawley (SD) rats. The wound closure outcomes revealed that B-2Ta effectively restrained the bacterial infection at a dose of 10 times minimal inhibitory concentration (MIC) during the 14 days of the wound healing process. Ultra-structure analyses showed that B-2Ta caused structural damage to the microorganism, and bacterial culture found that the number of microbes was significantly reduced by the end of treatment. Immunohistochemistry for the inflammatory marker IL-10 and the endothelial cell marker CD31 suggested positive effects on inflammatory status and epithelial migration and angiogenesis following treatment of the infected granulation tissues with B-2Ta. These results exhibited the continuous phase of inflammation reduction and wound healing acceleration in the B-2Ta-modulated re-epithelialisation of infected rats. Taken together, these data demonstrated that B-2Ta has great potential to be developed as antibacterial agents in clinic.

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References

Ochman H, Lawrence J, Groisman E . Lateral gene transfer and the nature of bacterial innovation. Nature. 2000; 405(6784):299-304. DOI: 10.1038/35012500. View

Cario E, Golenbock D, Visintin A, Runzi M, Gerken G, Podolsky D . Trypsin-sensitive modulation of intestinal epithelial MD-2 as mechanism of lipopolysaccharide tolerance. J Immunol. 2006; 176(7):4258-66. DOI: 10.4049/jimmunol.176.7.4258. View

Kiefer F, Arnold K, Kunzli M, Bordoli L, Schwede T . The SWISS-MODEL Repository and associated resources. Nucleic Acids Res. 2008; 37(Database issue):D387-92. PMC: 2686475. DOI: 10.1093/nar/gkn750. View

Simmaco M, Mignogna G, Barra D, Bossa F . Antimicrobial peptides from skin secretions of Rana esculenta. Molecular cloning of cDNAs encoding esculentin and brevinins and isolation of new active peptides. J Biol Chem. 1994; 269(16):11956-61. View

Zhao J, Zhao C, Liang G, Zhang M, Zheng J . Engineering antimicrobial peptides with improved antimicrobial and hemolytic activities. J Chem Inf Model. 2013; 53(12):3280-96. DOI: 10.1021/ci400477e. View

Arnold K, Bordoli L, Kopp J, Schwede T . The SWISS-MODEL workspace: a web-based environment for protein structure homology modelling. Bioinformatics. 2005; 22(2):195-201. DOI: 10.1093/bioinformatics/bti770. View

Greaves N, Ashcroft K, Baguneid M, Bayat A . Current understanding of molecular and cellular mechanisms in fibroplasia and angiogenesis during acute wound healing. J Dermatol Sci. 2013; 72(3):206-17. DOI: 10.1016/j.jdermsci.2013.07.008. View

Cavaillon J . Pro- versus anti-inflammatory cytokines: myth or reality. Cell Mol Biol (Noisy-le-grand). 2001; 47(4):695-702. View

Christofidou-Solomidou M, Nakada M, Williams J, Muller W, DeLisser H . Neutrophil platelet endothelial cell adhesion molecule-1 participates in neutrophil recruitment at inflammatory sites and is down-regulated after leukocyte extravasation. J Immunol. 1997; 158(10):4872-8. View

10.

Tada Y, Koarada S, Morito F, Ushiyama O, Haruta Y, Kanegae F . Acceleration of the onset of collagen-induced arthritis by a deficiency of platelet endothelial cell adhesion molecule 1. Arthritis Rheum. 2003; 48(11):3280-90. DOI: 10.1002/art.11268. View

11.

Lin Y, Hu N, Lyu P, Ma J, Wang L, Zhou M . Hylaranins: prototypes of a new class of amphibian antimicrobial peptide from the skin secretion of the oriental broad-folded frog, Hylarana latouchii. Amino Acids. 2014; 46(4):901-9. DOI: 10.1007/s00726-013-1655-1. View

12.

Rudin W, Favre N, Bordmann G, Ryffel B . Interferon-gamma is essential for the development of cerebral malaria. Eur J Immunol. 1997; 27(4):810-5. DOI: 10.1002/eji.1830270403. View

13.

Xu Z, Wang F, Fan F, Gu Y, Shan N, Meng X . Quantitative Proteomics Reveals That the Inhibition of Na(+)/K(+)-ATPase Activity Affects S-Phase Progression Leading to a Chromosome Segregation Disorder by Attenuating the Aurora A Function in Hepatocellular Carcinoma Cells. J Proteome Res. 2015; 14(11):4594-602. DOI: 10.1021/acs.jproteome.5b00724. View

14.

Biasini M, Bienert S, Waterhouse A, Arnold K, Studer G, Schmidt T . SWISS-MODEL: modelling protein tertiary and quaternary structure using evolutionary information. Nucleic Acids Res. 2014; 42(Web Server issue):W252-8. PMC: 4086089. DOI: 10.1093/nar/gku340. View

15.

Zhou M, Chen T, Walker B, Shaw C . Pelophylaxins: novel antimicrobial peptide homologs from the skin secretion of the Fukien gold-striped pond frog, Pelophylax plancyi fukienensis: identification by "shotgun" cDNA cloning and sequence analysis. Peptides. 2005; 27(1):36-41. DOI: 10.1016/j.peptides.2005.07.007. View

16.

Medzhitov R . Inflammation 2010: new adventures of an old flame. Cell. 2010; 140(6):771-6. DOI: 10.1016/j.cell.2010.03.006. View

17.

Kahn H, Marks A . A new monoclonal antibody, D2-40, for detection of lymphatic invasion in primary tumors. Lab Invest. 2002; 82(9):1255-7. DOI: 10.1097/01.lab.0000028824.03032.ab. View

18.

Ram M, Singh V, Kumawat S, Kant V, Tandan S, Kumar D . Bilirubin modulated cytokines, growth factors and angiogenesis to improve cutaneous wound healing process in diabetic rats. Int Immunopharmacol. 2015; 30:137-149. DOI: 10.1016/j.intimp.2015.11.037. View

19.

Wu Y, Wang L, Lin C, Lin Y, Zhou M, Chen L . Vasorelaxin: a novel arterial smooth muscle-relaxing eicosapeptide from the skin secretion of the Chinese piebald odorous frog (Odorrana schmackeri). PLoS One. 2013; 8(2):e55739. PMC: 3566010. DOI: 10.1371/journal.pone.0055739. View

20.

Mohamed M, Hammac G, Guptill L, Seleem M . Antibacterial activity of novel cationic peptides against clinical isolates of multi-drug resistant Staphylococcus pseudintermedius from infected dogs. PLoS One. 2015; 9(12):e116259. PMC: 4281220. DOI: 10.1371/journal.pone.0116259. View