Biosynthesis of Zinc Oxide Nanoparticles from (L.) Extract to Overcome Carbapenem-Resistant

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2021 Apr 3

PMID 33805514

Citations 18

Authors

Elsayim Rasha

AlOthman Monerah

Alkhulaifi Manal

Ali Rehab

Doud Mohammed

Elnagar Doaa

Affiliations

Soon will be listed here.

Abstract

Recently, concerns have been raised globally about antimicrobial resistance, the prevalence of which has increased significantly. Carbapenem-resistant (KPC) is considered one of the most common resistant bacteria, which has spread to ICUs in Saudi Arabia. This study was established to investigate the antibacterial activity of biosynthesized zinc oxide nanoparticles (ZnO-NPs) against KPC in vitro and in vivo. In this study, we used the aqueous extract of (L.) fruits to mediate the synthesis of ZnO-NPs. The nanoparticles produced were characterized by UV-vis spectroscopy, zetasizer and zeta potential analyses, X-ray diffraction (XRD) spectroscopy, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), and transmission electron microscopy (TEM). The antimicrobial activity of ZnO-NPs against KPC was determined via the well diffusion method, and determining minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), the results showed low MIC and MBC when compared with the MIC and MBC of Imipenem and Meropenem antibiotics. The results of in vitro analysis were supported by the results upon applying ZnO-NP ointment to promote wound closure of rats, which showed better wound healing than the results with imipenem ointment. The biosynthesized ZnO-NPs showed good potential for use against bacteria due to their small size, applicability, and low toxicity to human cells.

Citing Articles

Green synthesized ZnO and ZnO-based composites for wound healing applications.

Wafi A, Khan M Bioprocess Biosyst Eng. 2024; .

PMID: 39739126 DOI: 10.1007/s00449-024-03123-z.

Proclaiming Plant Growth-Promoting and Antifungal Properties of Pseudomonas lurida and Bacillus velezensis Isolated from Rhododendrons of Darjeeling Hills.

Biswas S, Chatterjee R, Rai U, Jana S, Mukhopadhyay M Curr Microbiol. 2024; 81(11):393.

PMID: 39369364 DOI: 10.1007/s00284-024-03900-9.

Assessing anticancer, antidiabetic, and antioxidant capacities in green-synthesized zinc oxide nanoparticles and solvent-based plant extracts.

Azeem M, Siddique M, Imran M, Zubair M, Mumtaz R, Younas M Heliyon. 2024; 10(14):e34073.

PMID: 39092244 PMC: 11292250. DOI: 10.1016/j.heliyon.2024.e34073.

Natural antibiotics against antimicrobial resistance: sources and bioinspired delivery systems.

Guedes B, Krambeck K, Durazzo A, Lucarini M, Santini A, Oliveira M Braz J Microbiol. 2024; 55(3):2753-2766.

PMID: 38888693 PMC: 11405619. DOI: 10.1007/s42770-024-01410-1.

Antifungal activity of fruit extract against : and study.

Aljuhani S, Rizwana H, Aloufi A, Alkahtani S, Albasher G, Almasoud H Front Microbiol. 2024; 15:1399671.

PMID: 38803379 PMC: 11128596. DOI: 10.3389/fmicb.2024.1399671.

References

Rao T, Riyazuddin , Babji P, Ahmad N, Khan R, Hassan I . Green synthesis and structural classification of mediated-silver doped titanium oxide (Ag/TiO) spherical nanoparticles: Assessment of its antimicrobial and anticancer activity. Saudi J Biol Sci. 2019; 26(7):1385-1391. PMC: 6904800. DOI: 10.1016/j.sjbs.2019.09.005. View

Ghasemi F, Jalal R . Antimicrobial action of zinc oxide nanoparticles in combination with ciprofloxacin and ceftazidime against multidrug-resistant Acinetobacter baumannii. J Glob Antimicrob Resist. 2016; 6:118-122. DOI: 10.1016/j.jgar.2016.04.007. View

Chen P, Seth A, Abercrombie J, Mustoe T, Leung K . Activity of imipenem against Klebsiella pneumoniae biofilms in vitro and in vivo. Antimicrob Agents Chemother. 2013; 58(2):1208-13. PMC: 3910830. DOI: 10.1128/AAC.01353-13. View

Soleymanzadeh Moghadam S, Mohammad N, Ghooshchian M, FathiZadeh S, Khodaii Z, Faramarzi M . Comparison of the effects of versus imipenem on infected burn wound healing. Med J Islam Repub Iran. 2020; 34:94. PMC: 7722975. DOI: 10.34171/mjiri.34.94. View

Sharma N, Singh V, Pandey A, Mishra B, Kulsoom M, Dasgupta N . Preparation and Evaluation of the ZnO NP-Ampicillin/Sulbactam Nanoantibiotic: Optimization of Formulation Variables Using RSM Coupled GA Method and Antibacterial Activities. Biomolecules. 2019; 9(12). PMC: 6995637. DOI: 10.3390/biom9120764. View

Aldalbahi A, Alterary S, Ali Abdullrahman Almoghim R, Awad M, Aldosari N, Fahad Alghannam S . Greener Synthesis of Zinc Oxide Nanoparticles: Characterization and Multifaceted Applications. Molecules. 2020; 25(18). PMC: 7571220. DOI: 10.3390/molecules25184198. View

Kim K, Choi M, Lee J, Jeong J, Kim Y, Kim M . Physicochemical properties of surface charge-modified ZnO nanoparticles with different particle sizes. Int J Nanomedicine. 2015; 9 Suppl 2:41-56. PMC: 4279853. DOI: 10.2147/IJN.S57923. View

Alekish M, Ismail Z, Albiss B, Nawasrah S . antibacterial effects of zinc oxide nanoparticles on multiple drug-resistant strains of and : An alternative approach for antibacterial therapy of mastitis in sheep. Vet World. 2018; 11(10):1428-1432. PMC: 6247879. DOI: 10.14202/vetworld.2018.1428-1432. View

Siddiquah A, Hashmi S, Mushtaq S, Renouard S, Blondeau J, Abbasi R . Exploiting potential and characterization of surface modified Zinc oxide nanoparticles of extract: Their clinical potential towards HepG2 cell line and human pathogenic bacteria. EXCLI J. 2018; 17:671-687. PMC: 6088224. DOI: 10.17179/excli2018-1327. View

10.

Zowawi H, Sartor A, Balkhy H, Walsh T, Al Johani S, Aljindan R . Molecular characterization of carbapenemase-producing Escherichia coli and Klebsiella pneumoniae in the countries of the Gulf cooperation council: dominance of OXA-48 and NDM producers. Antimicrob Agents Chemother. 2014; 58(6):3085-90. PMC: 4068443. DOI: 10.1128/AAC.02050-13. View

11.

Kelly A, Mathema B, Larson E . Carbapenem-resistant Enterobacteriaceae in the community: a scoping review. Int J Antimicrob Agents. 2017; 50(2):127-134. PMC: 5726257. DOI: 10.1016/j.ijantimicag.2017.03.012. View

12.

Liao J, Xu G, Mevers E, Clardy J, Watnick P . A high-throughput, whole cell assay to identify compounds active against carbapenem-resistant Klebsiella pneumoniae. PLoS One. 2018; 13(12):e0209389. PMC: 6303040. DOI: 10.1371/journal.pone.0209389. View

13.

Gao Y, Han Y, Cui M, Tey H, Wang L, Xu C . ZnO nanoparticles as an antimicrobial tissue adhesive for skin wound closure. J Mater Chem B. 2020; 5(23):4535-4541. DOI: 10.1039/c7tb00664k. View

14.

Tiwari V, Mishra N, Gadani K, Solanki P, Shah N, Tiwari M . Mechanism of Anti-bacterial Activity of Zinc Oxide Nanoparticle Against Carbapenem-Resistant . Front Microbiol. 2018; 9:1218. PMC: 5997932. DOI: 10.3389/fmicb.2018.01218. View

15.

DeLeo F, Chen L, Porcella S, Martens C, Kobayashi S, Porter A . Molecular dissection of the evolution of carbapenem-resistant multilocus sequence type 258 Klebsiella pneumoniae. Proc Natl Acad Sci U S A. 2014; 111(13):4988-93. PMC: 3977278. DOI: 10.1073/pnas.1321364111. View

16.

Zhang R, Liu L, Zhou H, Chan E, Li J, Fang Y . Nationwide Surveillance of Clinical Carbapenem-resistant Enterobacteriaceae (CRE) Strains in China. EBioMedicine. 2017; 19:98-106. PMC: 5440625. DOI: 10.1016/j.ebiom.2017.04.032. View

17.

Zhang Y, Nayak T, Hong H, Cai W . Biomedical applications of zinc oxide nanomaterials. Curr Mol Med. 2013; 13(10):1633-45. PMC: 3838497. DOI: 10.2174/1566524013666131111130058. View

18.

Yoshioka C, Ito Y, Nagai N . Enhanced percutaneous absorption of cilostazol nanocrystals using aqueous gel patch systems and clarification of the absorption mechanism. Exp Ther Med. 2018; 15(4):3501-3508. PMC: 5840931. DOI: 10.3892/etm.2018.5820. View

19.

Liu S, Long Q, Xu Y, Wang J, Xu Z, Wang L . Assessment of antimicrobial and wound healing effects of Brevinin-2Ta against the bacterium in dermally-wounded rats. Oncotarget. 2018; 8(67):111369-111385. PMC: 5762328. DOI: 10.18632/oncotarget.22797. View

20.

Hameed A, Karthikeyan C, Ahamed A, Thajuddin N, Alharbi N, Alharbi S . In vitro antibacterial activity of ZnO and Nd doped ZnO nanoparticles against ESBL producing Escherichia coli and Klebsiella pneumoniae. Sci Rep. 2016; 6:24312. PMC: 4829841. DOI: 10.1038/srep24312. View