A Knock-in Mouse Strain Facilitates Dynamic Tracking and Enrichment of MEIS1

Overview

Journal Blood Adv

Specialty Hematology

Date 2018 Jan 4

PMID 29296870

Citations 5

Authors

Ping Xiang

Wei Wei

Nicole Hofs

Jack Clemans-Gibbon

Tobias Maetzig

Courteney K Lai

Ishpreet Dhillon

Christopher May

Jens Ruschmann

Edith Schneider

Patricia Rosten

Kaiji Hu

Florian Kuchenbauer

Pamela A Hoodless

R Keith Humphries

Affiliations

Soon will be listed here.

Abstract

Myeloid ecotropic viral integration site 1 (MEIS1), a HOX transcription cofactor, is a critical regulator of normal hematopoiesis, and its overexpression is implicated in a wide range of leukemias. Using the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein-9 (Cas9) gene-editing system, we generated a knock-in transgenic mouse line in which a green fluorescent protein (GFP) reporter and a hemagglutinin (HA) epitope tag are inserted near the translational start site of endogenous . This novel reporter strain readily enables tracking of MEIS1 expression at single-cell-level resolution via the fluorescence reporter GFP, and facilitates MEIS1 detection and purification via the HA epitope tag. This new reporter mouse line provides powerful new approaches to track -expressing hematopoietic cells and to explore function and regulation during normal and leukemic hematopoiesis.

Citing Articles

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Elucidating the importance and regulation of key enhancers for human MEIS1 expression.

Xiang P, Yang X, Escano L, Dhillon I, Schneider E, Clemans-Gibbon J Leukemia. 2022; 36(8):1980-1989.

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