SLAM Family Markers Are Conserved Among Hematopoietic Stem Cells from Old and Reconstituted Mice and Markedly Increase Their Purity

Overview

Journal Blood

Publisher Elsevier

Specialty Hematology

Date 2005 Oct 13

PMID 16219798

Citations 125

Authors

Omer H Yilmaz

Mark J Kiel

Sean J Morrison

Affiliations

Soon will be listed here.

Abstract

Recent advances have increased the purity of hematopoietic stem cells (HSCs) isolated from young mouse bone marrow. However, little attention has been paid to the purity of HSCs from other contexts. Although Thy-1 low Sca-1+ Lineage- c-kit+ cells from young bone marrow are highly enriched for HSCs (1 in 5 cells gives long-term multilineage reconstitution after transplantation into irradiated mice), the same population from old, reconstituted, or cytokine-mobilized mice engrafts much less efficiently (1 in 78 to 1 in 185 cells gives long-term multilineage reconstitution). To test whether we could increase the purity of HSCs isolated from these contexts, we examined the SLAM family markers CD150 and CD48. All detectable HSCs from old, reconstituted, and cyclophosphamide/G-CSF-mobilized mice were CD150+ CD48-, just as in normal young bone marrow. Thy-1 low Sca-1+ Lineage- c-kit+ cells from old, reconstituted, or mobilized mice included mainly CD48+ and/or CD150- cells that lacked reconstituting ability. CD150+ CD48- Sca-1+ Lineage- c-kit+ cells from old, reconstituted, or mobilized mice were much more highly enriched for HSCs, with 1 in 3 to 1 in 7 cells giving long-term multilineage reconstitution. SLAM family receptor expression is conserved among HSCs from diverse contexts, and HSCs from old, reconstituted, and mobilized mice engraft relatively efficiently after transplantation when contaminating cells are eliminated.

Citing Articles

Hematopoietic stem cell discovery: unveiling the historical and future perspective of colony-forming units assay.

Yusoff N, Abd Hamid Z, Budin S, Taib I PeerJ. 2025; 13:e18854.

PMID: 39897489 PMC: 11786707. DOI: 10.7717/peerj.18854.

Therapeutic role of PTEN in tissue regeneration for management of neurological disorders: stem cell behaviors to an in-depth review.

Li Y, Ma R, Hao X Cell Death Dis. 2024; 15(4):268.

PMID: 38627382 PMC: 11021430. DOI: 10.1038/s41419-024-06657-y.

Postnatal development of mouse spermatogonial stem cells as determined by immunophenotype, regenerative capacity, and long-term culture-initiating ability: a model for practical applications.

Song Y, Zhang X, Desmarais J, Nagano M Sci Rep. 2024; 14(1):2299.

PMID: 38280889 PMC: 10821885. DOI: 10.1038/s41598-024-52824-8.

CK2β Regulates Hematopoietic Stem Cell Biology and Erythropoiesis.

Quotti Tubi L, Canovas Nunes S, Mandato E, Pizzi M, Vitulo N, DAgnolo M Hemasphere. 2023; 7(12):e978.

PMID: 38026791 PMC: 10673422. DOI: 10.1097/HS9.0000000000000978.

mTORC2 orchestrates monocytic and granulocytic lineage commitment by an ATF5-mediated pathway.

Zhao Y, Zhao C, Guo H, Zhang Z, Xu H, Shi M iScience. 2023; 26(9):107540.

PMID: 37649699 PMC: 10462862. DOI: 10.1016/j.isci.2023.107540.

References

Weissman I . Translating stem and progenitor cell biology to the clinic: barriers and opportunities. Science. 2000; 287(5457):1442-6. DOI: 10.1126/science.287.5457.1442. View

Chen C, Li L, Li M, Lodish H . The endoglin(positive) sca-1(positive) rhodamine(low) phenotype defines a near-homogeneous population of long-term repopulating hematopoietic stem cells. Immunity. 2003; 19(4):525-33. DOI: 10.1016/s1074-7613(03)00265-6. View

Christensen J, Weissman I . Flk-2 is a marker in hematopoietic stem cell differentiation: a simple method to isolate long-term stem cells. Proc Natl Acad Sci U S A. 2001; 98(25):14541-6. PMC: 64718. DOI: 10.1073/pnas.261562798. View

Wagers A, Allsopp R, Weissman I . Changes in integrin expression are associated with altered homing properties of Lin(-/lo)Thy1.1(lo)Sca-1(+)c-kit(+) hematopoietic stem cells following mobilization by cyclophosphamide/granulocyte colony-stimulating factor. Exp Hematol. 2002; 30(2):176-85. DOI: 10.1016/s0301-472x(01)00777-9. View

Uchida N, Dykstra B, Lyons K, Leung F, Eaves C . Different in vivo repopulating activities of purified hematopoietic stem cells before and after being stimulated to divide in vitro with the same kinetics. Exp Hematol. 2003; 31(12):1338-47. DOI: 10.1016/j.exphem.2003.09.001. View

Ema H, Nakauchi H . "Homing to Niche," a new criterion for hematopoietic stem cells?. Immunity. 2004; 20(1):1-2. DOI: 10.1016/s1074-7613(04)00002-0. View

Matsuzaki Y, Kinjo K, Mulligan R, Okano H . Unexpectedly efficient homing capacity of purified murine hematopoietic stem cells. Immunity. 2004; 20(1):87-93. DOI: 10.1016/s1074-7613(03)00354-6. View

Takano H, Ema H, Sudo K, Nakauchi H . Asymmetric division and lineage commitment at the level of hematopoietic stem cells: inference from differentiation in daughter cell and granddaughter cell pairs. J Exp Med. 2004; 199(3):295-302. PMC: 2211802. DOI: 10.1084/jem.20030929. View

Arai F, Hirao A, Ohmura M, Sato H, Matsuoka S, Takubo K . Tie2/angiopoietin-1 signaling regulates hematopoietic stem cell quiescence in the bone marrow niche. Cell. 2004; 118(2):149-61. DOI: 10.1016/j.cell.2004.07.004. View

10.

Venezia T, Merchant A, Ramos C, Whitehouse N, Young A, Shaw C . Molecular signatures of proliferation and quiescence in hematopoietic stem cells. PLoS Biol. 2004; 2(10):e301. PMC: 520599. DOI: 10.1371/journal.pbio.0020301. View

11.

Smith L, Weissman I, Heimfeld S . Clonal analysis of hematopoietic stem-cell differentiation in vivo. Proc Natl Acad Sci U S A. 1991; 88(7):2788-92. PMC: 51324. DOI: 10.1073/pnas.88.7.2788. View

12.

Uchida N, Weissman I . Searching for hematopoietic stem cells: evidence that Thy-1.1lo Lin- Sca-1+ cells are the only stem cells in C57BL/Ka-Thy-1.1 bone marrow. J Exp Med. 1992; 175(1):175-84. PMC: 2119064. DOI: 10.1084/jem.175.1.175. View

13.

Spangrude G, Brooks D, Tumas D . Long-term repopulation of irradiated mice with limiting numbers of purified hematopoietic stem cells: in vivo expansion of stem cell phenotype but not function. Blood. 1995; 85(4):1006-16. View

14.

Morrison S, Weissman I . The long-term repopulating subset of hematopoietic stem cells is deterministic and isolatable by phenotype. Immunity. 1994; 1(8):661-73. DOI: 10.1016/1074-7613(94)90037-x. View

15.

Morrison S, Hemmati H, Wandycz A, Weissman I . The purification and characterization of fetal liver hematopoietic stem cells. Proc Natl Acad Sci U S A. 1995; 92(22):10302-6. PMC: 40784. DOI: 10.1073/pnas.92.22.10302. View

16.

Morrison S, Uchida N, Weissman I . The biology of hematopoietic stem cells. Annu Rev Cell Dev Biol. 1995; 11:35-71. DOI: 10.1146/annurev.cb.11.110195.000343. View

17.

Osawa M, Hanada K, Hamada H, Nakauchi H . Long-term lymphohematopoietic reconstitution by a single CD34-low/negative hematopoietic stem cell. Science. 1996; 273(5272):242-5. DOI: 10.1126/science.273.5272.242. View

18.

Bodine D, Seidel N, Orlic D . Bone marrow collected 14 days after in vivo administration of granulocyte colony-stimulating factor and stem cell factor to mice has 10-fold more repopulating ability than untreated bone marrow. Blood. 1996; 88(1):89-97. View

19.

Wagers A, Sherwood R, Christensen J, Weissman I . Little evidence for developmental plasticity of adult hematopoietic stem cells. Science. 2002; 297(5590):2256-9. DOI: 10.1126/science.1074807. View

20.

Morrison S, Wandycz A, Akashi K, Globerson A, Weissman I . The aging of hematopoietic stem cells. Nat Med. 1996; 2(9):1011-6. DOI: 10.1038/nm0996-1011. View