Molecular Cloning of Feline Immunodeficiency Virus

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1989 Apr 1

PMID 2928341

Citations 77

Authors

R A Olmsted

A K Barnes

J K Yamamoto

V M Hirsch

R H Purcell

P R Johnson

Affiliations

Soon will be listed here.

Abstract

Feline immunodeficiency virus (FIV) is a T-lymphotropic retrovirus associated with immunodeficiency and opportunistic infections in cats. The discovery of FIV provides an opportunity for the development of a small animal model for AIDS. To initiate the molecular and biological characterization of FIV, cDNA clones were synthesized and used to isolate a proviral clone of FIV. Molecular cross-hybridization analysis of FIV with five lentiviruses revealed that nucleotide-sequence similarities exist between FIV and these lentiviruses in the gag-pol genes. However, nucleotide-sequence similarities were not seen upon comparison of the FIV long terminal repeat sequence with known viral sequences. Common antigenic determinants appeared to be shared by FIV, caprine arthritis encephalitis virus, and visna virus as shown by serological cross-reactivity of rabbit antibodies to caprine arthritis encephalitis virus and visna virus with the putative FIV core protein p28. These studies demonstrated that FIV is a member of the lentivirus subfamily and is distantly related to the AIDS lentiviruses of primates. Importantly, progeny virions of our molecular clone were infectious for experimentally inoculated cats. The availability of an infectious molecular clone will make possible a detailed dissection of the molecular pathogenesis of FIV, which may facilitate the development of vaccine and therapeutic strategies for AIDS.

Citing Articles

Identification of a putative Gag binding site critical for feline immunodeficiency virus genomic RNA packaging.

Krishnan A, Ali L, Prabhu S, Pillai V, Chameettachal A, Vivet-Boudou V RNA. 2023; 30(1):68-88.

PMID: 37914398 PMC: 10726167. DOI: 10.1261/rna.079840.123.

Mechanisms underlying HIV-associated cognitive impairment and emerging therapies for its management.

Ellis R, Marquine M, Kaul M, Fields J, Schlachetzki J Nat Rev Neurol. 2023; 19(11):668-687.

PMID: 37816937 PMC: 11052664. DOI: 10.1038/s41582-023-00879-y.

Gene Therapy Applications of Non-Human Lentiviral Vectors.

Munis A Viruses. 2020; 12(10).

PMID: 33003635 PMC: 7599719. DOI: 10.3390/v12101106.

Methamphetamine and Cannabis: A Tale of Two Drugs and their Effects on HIV, Brain, and Behavior.

Saloner R, Fields J, Marcondes M, Iudicello J, von Kanel S, Cherner M J Neuroimmune Pharmacol. 2020; 15(4):743-764.

PMID: 32929575 PMC: 7719083. DOI: 10.1007/s11481-020-09957-0.

Purification and Functional Characterization of a Biologically Active Full-Length Feline Immunodeficiency Virus (FIV) Pr50.

Krishnan A, Pillai V, Chameettachal A, Ali L, Pitchai F, Tariq S Viruses. 2019; 11(8).

PMID: 31357656 PMC: 6723490. DOI: 10.3390/v11080689.

References

Starcich B, Ratner L, Josephs S, Okamoto T, Gallo R, Wong-Staal F . Characterization of long terminal repeat sequences of HTLV-III. Science. 1985; 227(4686):538-40. DOI: 10.1126/science.2981438. View

Gubler U, HOFFMAN B . A simple and very efficient method for generating cDNA libraries. Gene. 1983; 25(2-3):263-9. DOI: 10.1016/0378-1119(83)90230-5. View

Birnstiel M, Busslinger M, Strub K . Transcription termination and 3' processing: the end is in site!. Cell. 1985; 41(2):349-59. DOI: 10.1016/s0092-8674(85)80007-6. View

Sonigo P, Alizon M, Staskus K, Klatzmann D, Cole S, Danos O . Nucleotide sequence of the visna lentivirus: relationship to the AIDS virus. Cell. 1985; 42(1):369-82. DOI: 10.1016/s0092-8674(85)80132-x. View

Fisher A, Collalti E, Ratner L, Gallo R, Wong-Staal F . A molecular clone of HTLV-III with biological activity. Nature. 1985; 316(6025):262-5. DOI: 10.1038/316262a0. View

Stephens R, Casey J, Rice N . Equine infectious anemia virus gag and pol genes: relatedness to visna and AIDS virus. Science. 1986; 231(4738):589-94. DOI: 10.1126/science.3003905. View

Pyper J, Clements J, Gonda M, Narayan O . Sequence homology between cloned caprine arthritis encephalitis virus and visna virus, two neurotropic lentiviruses. J Virol. 1986; 58(2):665-70. PMC: 252960. DOI: 10.1128/JVI.58.2.665-670.1986. View

Gonda M, Braun M, Clements J, Pyper J, Wong-Staal F, Gallo R . Human T-cell lymphotropic virus type III shares sequence homology with a family of pathogenic lentiviruses. Proc Natl Acad Sci U S A. 1986; 83(11):4007-11. PMC: 323654. DOI: 10.1073/pnas.83.11.4007. View

Riedel N, Hoover E, Gasper P, Nicolson M, Mullins J . Molecular analysis and pathogenesis of the feline aplastic anemia retrovirus, feline leukemia virus C-Sarma. J Virol. 1986; 60(1):242-50. PMC: 253922. DOI: 10.1128/JVI.60.1.242-250.1986. View

10.

Feinberg M, Jarrett R, Aldovini A, Gallo R, Wong-Staal F . HTLV-III expression and production involve complex regulation at the levels of splicing and translation of viral RNA. Cell. 1986; 46(6):807-17. DOI: 10.1016/0092-8674(86)90062-0. View

11.

Hess J, Pyper J, Clements J . Nucleotide sequence and transcriptional activity of the caprine arthritis-encephalitis virus long terminal repeat. J Virol. 1986; 60(2):385-93. PMC: 288904. DOI: 10.1128/JVI.60.2.385-393.1986. View

12.

Sen R, Baltimore D . Inducibility of kappa immunoglobulin enhancer-binding protein Nf-kappa B by a posttranslational mechanism. Cell. 1986; 47(6):921-8. DOI: 10.1016/0092-8674(86)90807-x. View

13.

Derse D, Dorn P, Levy L, Stephens R, Rice N, Casey J . Characterization of equine infectious anemia virus long terminal repeat. J Virol. 1987; 61(3):743-7. PMC: 254015. DOI: 10.1128/JVI.61.3.743-747.1987. View

14.

Pedersen N, Ho E, Brown M, Yamamoto J . Isolation of a T-lymphotropic virus from domestic cats with an immunodeficiency-like syndrome. Science. 1987; 235(4790):790-3. DOI: 10.1126/science.3643650. View

15.

Hirsch V, Riedel N, Mullins J . The genome organization of STLV-3 is similar to that of the AIDS virus except for a truncated transmembrane protein. Cell. 1987; 49(3):307-19. DOI: 10.1016/0092-8674(87)90283-2. View

16.

Kawakami T, Sherman L, Dahlberg J, Gazit A, Yaniv A, Tronick S . Nucleotide sequence analysis of equine infectious anemia virus proviral DNA. Virology. 1987; 158(2):300-12. DOI: 10.1016/0042-6822(87)90202-9. View

17.

Willey R, Smith D, Lasky L, THEODORE T, Earl P, Moss B . In vitro mutagenesis identifies a region within the envelope gene of the human immunodeficiency virus that is critical for infectivity. J Virol. 1988; 62(1):139-47. PMC: 250512. DOI: 10.1128/JVI.62.1.139-147.1988. View

18.

Gonda M, Braun M, Carter S, Kost T, Bess Jr J, Arthur L . Characterization and molecular cloning of a bovine lentivirus related to human immunodeficiency virus. Nature. 1987; 330(6146):388-91. DOI: 10.1038/330388a0. View

19.

Yamamoto J, Sparger E, Ho E, Andersen P, OConnor T, Mandell C . Pathogenesis of experimentally induced feline immunodeficiency virus infection in cats. Am J Vet Res. 1988; 49(8):1246-58. View

20.

Yamamoto J, Hansen H, Ho E, Morishita T, Okuda T, Sawa T . Epidemiologic and clinical aspects of feline immunodeficiency virus infection in cats from the continental United States and Canada and possible mode of transmission. J Am Vet Med Assoc. 1989; 194(2):213-20. View