» Articles » PMID: 29276501

Host and Pathway Engineering for Enhanced Lycopene Biosynthesis in

Overview
Journal Front Microbiol
Specialty Microbiology
Date 2017 Dec 26
PMID 29276501
Citations 32
Authors
Affiliations
Soon will be listed here.
Abstract

Carotenoids are a class of molecules with commercial value as food and feed additives with nutraceutical properties. Shifting carotenoid synthesis from petrochemical-based precursors to bioproduction from sugars and other biorenewable carbon sources promises to improve process sustainability and economics. In this work, we engineered the oleaginous yeast to produce the carotenoid lycopene. To enhance lycopene production, we tested a series of strategies to modify host cell physiology and metabolism, the most successful of which were mevalonate pathway overexpression and alleviating auxotrophies previously engineered into the PO1f strain of . The beneficial engineering strategies were combined into a single strain, which was then cultured in a 1-L bioreactor to produce 21.1 mg/g DCW. The optimized strain overexpressed a total of eight genes including two copies of HMG1, two copies of CrtI, and single copies of MVD1, EGR8, CrtB, and CrtE. Recovering leucine and uracil biosynthetic capacity also produced significant enhancement in lycopene titer. The successful engineering strategies characterized in this work represent a significant increase in understanding carotenoid biosynthesis in , not only increasing lycopene titer but also informing future studies on carotenoid biosynthesis.

Citing Articles

From the depths of the Java Trench: genomic analysis of Priestia flexa JT4 reveals bioprospecting and lycopene production potential.

Radjasa O, Steven R, Natanael Y, Nugrahapraja H, Radjasa S, Kristianti T BMC Genomics. 2024; 25(1):1259.

PMID: 39736538 PMC: 11687134. DOI: 10.1186/s12864-024-11115-2.


Progress in the Metabolic Engineering of for the Synthesis of Terpenes.

Liu S, Xu L, Sun Y, Yuan L, Xu H, Song X Biodes Res. 2024; 6:0051.

PMID: 39534575 PMC: 11555184. DOI: 10.34133/bdr.0051.


Construction of a Calibration Curve for Lycopene on a Liquid-Handling Platform─Wider Lessons for the Development of Automated Dilution Protocols.

Bultelle M, Casas A, Kitney R ACS Synth Biol. 2024; 13(8):2357-2375.

PMID: 39096303 PMC: 11334188. DOI: 10.1021/acssynbio.4c00031.


Genome Editing, Transcriptional Regulation, and Forward Genetic Screening Using CRISPR-Cas12a Systems in Yarrowia lipolytica.

Ramesh A, Lee S, Wheeldon I Methods Mol Biol. 2024; 2760:169-198.

PMID: 38468089 DOI: 10.1007/978-1-0716-3658-9_11.


Engineering of for terpenoid production.

Arnesen J, Borodina I Metab Eng Commun. 2022; 15:e00213.

PMID: 36387772 PMC: 9663531. DOI: 10.1016/j.mec.2022.e00213.


References
1.
Armstrong G, Hearst J . Carotenoids 2: Genetics and molecular biology of carotenoid pigment biosynthesis. FASEB J. 1996; 10(2):228-37. DOI: 10.1096/fasebj.10.2.8641556. View

2.
Schwartz C, Hussain M, Blenner M, Wheeldon I . Synthetic RNA Polymerase III Promoters Facilitate High-Efficiency CRISPR-Cas9-Mediated Genome Editing in Yarrowia lipolytica. ACS Synth Biol. 2015; 5(4):356-9. DOI: 10.1021/acssynbio.5b00162. View

3.
Xie W, Ye L, Lv X, Xu H, Yu H . Sequential control of biosynthetic pathways for balanced utilization of metabolic intermediates in Saccharomyces cerevisiae. Metab Eng. 2014; 28:8-18. DOI: 10.1016/j.ymben.2014.11.007. View

4.
Rakicka M, Lazar Z, Dulermo T, Fickers P, Nicaud J . Lipid production by the oleaginous yeast Yarrowia lipolytica using industrial by-products under different culture conditions. Biotechnol Biofuels. 2015; 8:104. PMC: 4513389. DOI: 10.1186/s13068-015-0286-z. View

5.
Blazeck J, Liu L, Redden H, Alper H . Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Appl Environ Microbiol. 2011; 77(22):7905-14. PMC: 3208987. DOI: 10.1128/AEM.05763-11. View