Terpenoid Indole Alkaloid Biosynthesis in Catharanthus Roseus: Effects and Prospects of Environmental Factors in Metabolic Engineering

Overview

Journal Biotechnol Lett

Specialties Biochemistry
Biotechnology

Date 2021 Sep 26

PMID 34564757

Citations 9

Authors

Yongliang Liu

Barunava Patra

Sanjay Kumar Singh

Priyanka Paul

Yan Zhou

Yongqing Li

Ying Wang

Sitakanta Pattanaik

Ling Yuan

Affiliations

Soon will be listed here.

Abstract

Plants synthesize a vast array of specialized metabolites that primarily contribute to their defense and survival under adverse conditions. Many of the specialized metabolites have therapeutic values as drugs. Biosynthesis of specialized metabolites is affected by environmental factors including light, temperature, drought, salinity, and nutrients, as well as pathogens and insects. These environmental factors trigger a myriad of changes in gene expression at the transcriptional and posttranscriptional levels. The dynamic changes in gene expression are mediated by several regulatory proteins that perceive and transduce the signals, leading to up- or down-regulation of the metabolic pathways. Exploring the environmental effects and related signal cascades is a strategy in metabolic engineering to produce valuable specialized metabolites. However, mechanistic studies on environmental factors affecting specialized metabolism are limited. The medicinal plant Catharanthus roseus (Madagascar periwinkle) is an important source of bioactive terpenoid indole alkaloids (TIAs), including the anticancer therapeutics vinblastine and vincristine. The emerging picture shows that various environmental factors significantly alter TIA accumulation by affecting the expression of regulatory and enzyme-encoding genes in the pathway. Compared to our understanding of the TIA pathway in response to the phytohormone jasmonate, the impacts of environmental factors on TIA biosynthesis are insufficiently studied and discussed. This review thus focuses on these aspects and discusses possible strategies for metabolic engineering of TIA biosynthesis. PURPOSE OF WORK: Catharanthus roseus is a rich source of bioactive terpenoid indole alkaloids (TIAs). The objective of this work is to present a comprehensive account of the influence of various biotic and abiotic factors on TIA biosynthesis and to discuss possible strategies to enhance TIA production through metabolic engineering.

Citing Articles

Characterization of the ZCTs, a subgroup of Cys2-His2 zinc finger transcription factors regulating alkaloid biosynthesis in Catharanthus roseus.

Traverse K, Breselge S, Trautman J, Dee A, Wang J, Childs K Plant Cell Rep. 2024; 43(9):209.

PMID: 39115578 PMC: 11310244. DOI: 10.1007/s00299-024-03295-8.

The Integration of the Metabolome and Transcriptome for Lindl. in Response to Methyl Jasmonate.

Gong D, Li B, Wu B, Fu D, Li Z, Wei H Molecules. 2023; 28(23).

PMID: 38067620 PMC: 10707931. DOI: 10.3390/molecules28237892.

Reference Genes Screening and Gene Expression Patterns Analysis Involved in Gelsenicine Biosynthesis under Different Hormone Treatments in .

Zhang Y, Mu D, Wang L, Wang X, Wilson I, Chen W Int J Mol Sci. 2023; 24(21).

PMID: 37958955 PMC: 10648913. DOI: 10.3390/ijms242115973.

A near-complete genome assembly of Catharanthus roseus and insights into its vinblastine biosynthesis and high susceptibility to the Huanglongbing pathogen.

Xu Z, Wang G, Wang Q, Li X, Zhang G, Qurban A Plant Commun. 2023; 4(6):100661.

PMID: 37464741 PMC: 10721464. DOI: 10.1016/j.xplc.2023.100661.

Interference between ER stress-related bZIP-type and jasmonate-inducible bHLH-type transcription factors in the regulation of triterpene saponin biosynthesis in .

Ribeiro B, Erffelinck M, Lacchini E, Ceulemans E, Colinas M, Williams C Front Plant Sci. 2022; 13:903793.

PMID: 36247618 PMC: 9562455. DOI: 10.3389/fpls.2022.903793.

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