Theory About the Embryo Cryo-Treatment

Overview

Journal Reprod Med Biol

Specialty Biology

Date 2017 Dec 21

PMID 29259458

Citations 11

Authors

Iavor K Vladimirov

Desislava Tacheva

Antonio Diez

Affiliations

Soon will be listed here.

Abstract

Background: To create hypothesis, which can give a logical explanation related to the benefits of freezing/thawing embryos. Cryopreservation is not only a technology used for storing embryos, but also a method of embryo treatment that can potentially improve the success rate in infertile couples.

Methods: From the analysis of multiple results in assisted reproductive technology, which have no satisfactory explanation to date, we found evidence to support a 'therapeutic' effect of the freezing/thawing of embryos on the process of recovery of the embryo and its subsequent implantation.

Results: Freezing/thawing is a way to activate the endogenous survival and repair responses in preimplantation embryos. Several molecular mechanisms can explain the higher success rate of ET using thawed embryos compared to fresh ET in women of advanced reproductive age, the higher miscarriage rate in cases of thawed blastocyst ET compared to thawed ET at early cleavage embryo, and the higher perinatal parameters of born children after thawed ET. Embryo thawing induces a stress. Controlled stress is not necessarily detrimental, because it generates a phenomenon that is counteracted by several known biological responses aimed to repair mitochondrial damage of membrane and protein misfolding. The term for favorable biological responses to low exposures to stress is called hormesis.

Conclusions: This thesis will summarize the role of cryopreservation in the activation of a hormetic response, preserving the mitochondrial function, improving survival, and having an impact on the process of implantation, miscarriage, and the development of pregnancy.

Citing Articles

Evaluation of microRNA expression profiles in human sperm frozen using permeable cryoprotectant-free droplet vitrification and conventional methods.

Zhang L, Mao J, Zhou Y, Mao G, Guo R, Ge H Asian J Androl. 2024; 26(4):366-376.

PMID: 38738948 PMC: 11280198. DOI: 10.4103/aja202390.

Extending the culture of cleavage-stage embryos to the blastocyst stage after warming increases the chance of live birth: does it have a regenerative effect?.

Onalan G, Tunc M, Tohma A, Gunakan E, Eryilmaz T, Zeyneloglu H Arch Gynecol Obstet. 2023; 307(6):1969-1974.

PMID: 37022346 DOI: 10.1007/s00404-023-07031-7.

Time from trophectoderm biopsy to vitrification affects the developmental competence of biopsied blastocysts.

Miki T, Ezoe K, Kouraba S, Ohata K, Kato K Reprod Med Biol. 2022; 21(1):e12439.

PMID: 35386383 PMC: 8967302. DOI: 10.1002/rmb2.12439.

The clinical outcomes of fresh versus frozen embryos transfer in women ≥40 years with poor ovarian response.

Liu C, Li Y, Jiang H, Liu Y, Song X Obstet Gynecol Sci. 2021; 64(3):284-292.

PMID: 33517605 PMC: 8138078. DOI: 10.5468/ogs.20124.

Higher efficiency of frozen embryo transfer in male infertility cases in in vitro fertilization.

Adanacioglu F, Tokat Z, Buyukfidan D, Ozgul H, Urunsak F, Cayan S Turk J Urol. 2020; 45(Supp. 1):S7-S12.

PMID: 33120002 PMC: 7595027. DOI: 10.5152/tud.2019.99422.

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