The Structural Basis for Dynamic DNA Binding and Bridging Interactions Which Condense the Bacterial Centromere

Overview

Journal Elife

Specialty Biology

Date 2017 Dec 16

PMID 29244022

Citations 34

Authors

Gemma Lm Fisher

Cesar L Pastrana

Victoria A Higman

Alan Koh

James A Taylor

Annika Butterer

Timothy Craggs

Frank Sobott

Heath Murray

Matthew P Crump

Fernando Moreno-Herrero

Mark S Dillingham

Affiliations

Soon will be listed here.

Abstract

The ParB protein forms DNA bridging interactions around to condense DNA and earmark the bacterial chromosome for segregation. The molecular mechanism underlying the formation of these ParB networks is unclear. We show here that while the central DNA binding domain is essential for anchoring at , this interaction is not required for DNA condensation. Structural analysis of the C-terminal domain reveals a dimer with a lysine-rich surface that binds DNA non-specifically and is essential for DNA condensation in vitro. Mutation of either the dimerisation or the DNA binding interface eliminates ParB-GFP foci formation in vivo. Moreover, the free C-terminal domain can rapidly decondense ParB networks independently of its ability to bind DNA. Our work reveals a dual role for the C-terminal domain of ParB as both a DNA binding and bridging interface, and highlights the dynamic nature of ParB networks in .

Citing Articles

Phase-separated ParB enforces diverse DNA compaction modes and stabilizes the parS-centered partition complex.

Zhao Y, Guo L, Hu J, Ren Z, Li Y, Hu M Nucleic Acids Res. 2024; 52(14):8385-8398.

PMID: 38908027 PMC: 11317135. DOI: 10.1093/nar/gkae533.

Insights into the molecular mechanism of ParABS system in chromosome partition by HpParA and HpParB.

Chu C, Wu C, Lin M, Yen C, Wu Y, Hsiao C Nucleic Acids Res. 2024; 52(12):7321-7336.

PMID: 38842933 PMC: 11229316. DOI: 10.1093/nar/gkae450.

The virulence regulator VirB from Shigella flexneri uses a CTP-dependent switch mechanism to activate gene expression.

Jakob S, Steinchen W, Hanssmann J, Rosum J, Langenfeld K, Osorio-Valeriano M Nat Commun. 2024; 15(1):318.

PMID: 38182620 PMC: 10770331. DOI: 10.1038/s41467-023-44509-z.

Connecting the dots: key insights on ParB for chromosome segregation from single-molecule studies.

Tisma M, Kaljevic J, Gruber S, Le T, Dekker C FEMS Microbiol Rev. 2023; 48(1).

PMID: 38142222 PMC: 10786196. DOI: 10.1093/femsre/fuad067.

VirB, a transcriptional activator of virulence in Shigella flexneri, uses CTP as a cofactor.

Antar H, Gruber S Commun Biol. 2023; 6(1):1204.

PMID: 38007587 PMC: 10676424. DOI: 10.1038/s42003-023-05590-8.

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