Engineering of Protein Nanopores for Sequencing, Chemical or Protein Sensing and Disease Diagnosis

Overview

Journal Curr Opin Biotechnol

Publisher Elsevier

Specialty Biotechnology

Date 2017 Dec 13

PMID 29232619

Citations 26

Authors

Shaoying Wang

Zhengyi Zhao

Farzin Haque

Peixuan Guo

Affiliations

Soon will be listed here.

Abstract

Biological systems contain highly-ordered structures performing diverse functions. The elegant structures of biomachines have inspired the development of nanopores as single molecule sensors. Over the years, the utility of nanopores for detecting a wide variety of analytes have rapidly emerged for sensing, sequencing and diagnostic applications. Several protein channels with diverse shapes and sizes, such as motor channels from bacteriophage Phi29, SPP1, T3, and T4, as well as α-hemolysin, MspA, aerolysin, FluA, OmpF/G, CsgG, ClyA, have been continually investigated and developed as nanopores. Herein, we focus on advances in biological nanopores for single molecule sensing and DNA sequencing from a protein engineering standpoint for changing pore sizes, altering charge distributions, enhancing sensitivity, improving stability, and imparting new detection capabilities.

Citing Articles

Hetero-Oligomeric Protein Pores for Single-Molecule Sensing.

Satheesan R, Janeena A, Mahendran K J Membr Biol. 2024; .

PMID: 39699641 DOI: 10.1007/s00232-024-00331-2.

The potential of nanopore technologies toward empowering biophysical research: Brief history, basic principle and applications.

Yamazaki H, Peng Z, Kawano R, Shoji K Biophys Physicobiol. 2024; 21(1):e210003.

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Nanopore Single-molecule Analysis of Biomarkers: Providing Possible Clues to Disease Diagnosis.

Chen X, Zhou S, Wang Y, Zheng L, Guan S, Wang D Trends Analyt Chem. 2023; 162.

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Structural bioinformatics studies of bacterial outer membrane beta-barrel transporters and their AlphaFold2 predicted water-soluble QTY variants.

Sajeev-Sheeja A, Smorodina E, Zhang S PLoS One. 2023; 18(8):e0290360.

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Gating of β-Barrel Protein Pores, Porins, and Channels: An Old Problem with New Facets.

Mayse L, Movileanu L Int J Mol Sci. 2023; 24(15).

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