MRNA Processing Factor CstF-50 and Ubiquitin Escort Factor P97 Are BRCA1/BARD1 Cofactors Involved in Chromatin Remodeling During the DNA Damage Response

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 2017 Nov 29

PMID 29180510

Citations 6

Authors

Danae Fonseca

Jorge Baquero

Michael R Murphy

Gamage Aruggoda

Sophia Varriano

Carmen Sapienza

Oksana Mashadova

Shadaqur Rahman

Frida E Kleiman

Affiliations

Soon will be listed here.

Abstract

The cellular response to DNA damage is an intricate mechanism that involves the interplay among several pathways. In this study, we provide evidence of the roles of the polyadenylation factor cleavage stimulation factor 50 (CstF-50) and the ubiquitin (Ub) escort factor p97 as cofactors of BRCA1/BARD1 E3 Ub ligase, facilitating chromatin remodeling during the DNA damage response (DDR). CstF-50 and p97 formed complexes with BRCA1/BARD1, Ub, and some BRCA1/BARD1 substrates, such as RNA polymerase (RNAP) II and histones. Furthermore, CstF-50 and p97 had an additive effect on the activation of the ubiquitination of these BRCA1/BARD1 substrates during DDR. Importantly, as a result of these functional interactions, BRCA1/BARD1/CstF-50/p97 had a specific effect on the chromatin structure of genes that were differentially expressed. This study provides new insights into the roles of RNA processing, BRCA1/BARD1, the Ub pathway, and chromatin structure during DDR.

Citing Articles

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