A Multiplex SYBR Green Real-Time PCR Assay for the Detection of Three Colistin Resistance Genes from Cultured Bacteria, Feces, and Environment Samples

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2017 Nov 23

PMID 29163387

Citations 25

Authors

Jiyun Li

Xiaomin Shi

Wenjuan Yin

Yang Wang

Zhangqi Shen

Shuangyang Ding

Shaolin Wang

Affiliations

Soon will be listed here.

Abstract

The aim of the study was to develop a multiplex assay for rapid detection of , and , a group of genes of conferring resistance to colistin mediated by plasmid in Enterobacteriaceae. A SYBR Green based real-time PCR assay has been designed to detect the genes, and applied to cultured bacteria, feces and soil samples. All three genes could be detected with a lower limit of 10 cultured bacteria. This test was highly specific and sensitive, and generated no false-positive results. The assay was also conclusive when applied to feces and soil samples containing -positive , which could facilitate the screening of genes not only in the bacteria, but also directly from the environment. This simple, rapid, sensitive, and specific multiplex assay will be useful for rapid screening of the colistin resistance in both clinical medicine and animal husbandry.

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