A G3P[13] Porcine Group A Rotavirus Emerging in China is a Reassortant and a Natural Recombinant in the VP4 Gene

Overview

Journal Transbound Emerg Dis

Specialty Veterinary Medicine

Date 2017 Nov 18

PMID 29148270

Citations 10

Authors

Z Jing

X Zhang

H Shi

J Chen

D Shi

H Dong

L Feng

Affiliations

Soon will be listed here.

Abstract

Group A rotaviruses (RVAs) are a major cause of serious intestinal disease in piglets. In this study, a novel pig strain was identified in a stool sample from China. The strain was designated RVA/Pig/China/LNCY/2016/G3P[13] and had a G3-P[13]-I5-R1-C1-M1-A8-N1-T1-E1-H1 genome. The viral protein 7 (VP7) and non-structural protein 4 (NSP4) genes of RVA/Pig/China/LNCY/2016/G3P[13] were closely related to cogent genes of human RVAs, suggesting that a reassortment between pig and human strains had occurred. Recombination analysis showed that RVA/Pig/China/LNCY/2016/G3P[13] is a natural recombinant strain between the P[23] and P[7] RVA strains, and crossover points for recombination were found at nucleotides (nt) 456 and 804 of the VP4 gene. Elucidating the biological characteristics of porcine rotavirus (PoRV) will be helpful for further analyses of the epidemic characteristics of this virus. The results of this study provide valuable information for RVA recombination and evolution and will facilitate future investigations into the molecular pathogenesis of RVAs.

Citing Articles

Complete Genome Classification System of : An Updated Analysis.

Diaz Alarcon R, Salvatierra K, Gomez Quintero E, Liotta D, Parreno V, Mino S Viruses. 2025; 17(2).

PMID: 40006966 PMC: 11860323. DOI: 10.3390/v17020211.

Identification and characterization of porcine Rotavirus A in Chilean swine population.

Neira V, Melgarejo C, Urzua-Encina C, Berrios F, Valdes V, Mor S Front Vet Sci. 2023; 10:1240346.

PMID: 38026647 PMC: 10652281. DOI: 10.3389/fvets.2023.1240346.

Triplex-Loop-Mediated Isothermal Amplification Combined with a Lateral Flow Immunoassay for the Simultaneous Detection of Three Pathogens of Porcine Viral Diarrhea Syndrome in Swine.

Hong Y, Ma B, Li J, Shuai J, Zhang X, Xu H Animals (Basel). 2023; 13(12).

PMID: 37370420 PMC: 10295087. DOI: 10.3390/ani13121910.

Genome Analysis of the G6P6 Genotype of Porcine Group C Rotavirus in China.

Jiao R, Ji Z, Zhu X, Shi H, Chen J, Shi D Animals (Basel). 2022; 12(21).

PMID: 36359075 PMC: 9657714. DOI: 10.3390/ani12212951.

Metagenomic analysis of viral diversity and a novel astroviruse of forest rodent.

Yin H, Wan D, Chen H Virol J. 2022; 19(1):138.

PMID: 36045380 PMC: 9429442. DOI: 10.1186/s12985-022-01847-6.

References

Banyai K, Esona M, Mijatovic S, Kerin T, Pedreira C, Mercado J . Zoonotic bovine rotavirus strain in a diarrheic child, Nicaragua. J Clin Virol. 2009; 46(4):391-3. DOI: 10.1016/j.jcv.2009.08.005. View

Patton J, Hua J, Mansell E . Location of intrachain disulfide bonds in the VP5* and VP8* trypsin cleavage fragments of the rhesus rotavirus spike protein VP4. J Virol. 1993; 67(8):4848-55. PMC: 237872. DOI: 10.1128/JVI.67.8.4848-4855.1993. View

Matthijnssens J, Otto P, Ciarlet M, Desselberger U, Van Ranst M, Johne R . VP6-sequence-based cutoff values as a criterion for rotavirus species demarcation. Arch Virol. 2012; 157(6):1177-82. DOI: 10.1007/s00705-012-1273-3. View

Phan T, Okitsu S, Maneekarn N, Ushijima H . Evidence of intragenic recombination in G1 rotavirus VP7 genes. J Virol. 2007; 81(18):10188-94. PMC: 2045391. DOI: 10.1128/JVI.00337-07. View

Matthijnssens J, Potgieter C, Ciarlet M, Parreno V, Martella V, Banyai K . Are human P[14] rotavirus strains the result of interspecies transmissions from sheep or other ungulates that belong to the mammalian order Artiodactyla?. J Virol. 2009; 83(7):2917-29. PMC: 2655590. DOI: 10.1128/JVI.02246-08. View

Singh Malik Y, Kumar N, Sharma K, Saurabh S, Dhama K, Prasad M . Multispecies reassortant bovine rotavirus strain carries a novel simian G3-like VP7 genotype. Infect Genet Evol. 2016; 41:63-72. DOI: 10.1016/j.meegid.2016.03.023. View

Mackow E, Shaw R, Matsui S, Vo P, Benfield D, Greenberg H . Characterization of homotypic and heterotypic VP7 neutralization sites of rhesus rotavirus. Virology. 1988; 165(2):511-7. DOI: 10.1016/0042-6822(88)90595-8. View

Suzuki Y, Gojobori T, Nakagomi O . Intragenic recombinations in rotaviruses. FEBS Lett. 1998; 427(2):183-7. DOI: 10.1016/s0014-5793(98)00415-3. View

Matthijnssens J, Ciarlet M, Heiman E, Arijs I, Delbeke T, McDonald S . Full genome-based classification of rotaviruses reveals a common origin between human Wa-Like and porcine rotavirus strains and human DS-1-like and bovine rotavirus strains. J Virol. 2008; 82(7):3204-19. PMC: 2268446. DOI: 10.1128/JVI.02257-07. View

10.

Hu L, Crawford S, Czako R, Cortes-Penfield N, Smith D, Le Pendu J . Cell attachment protein VP8* of a human rotavirus specifically interacts with A-type histo-blood group antigen. Nature. 2012; 485(7397):256-9. PMC: 3350622. DOI: 10.1038/nature10996. View

11.

Pesavento J, Crawford S, Estes M, Prasad B . Rotavirus proteins: structure and assembly. Curr Top Microbiol Immunol. 2006; 309:189-219. DOI: 10.1007/3-540-30773-7_7. View

12.

Jing Z, Zhang X, Shi H, Chen J, Shi D, Dong H . A G3P[13] porcine group A rotavirus emerging in China is a reassortant and a natural recombinant in the VP4 gene. Transbound Emerg Dis. 2017; 65(2):e317-e328. PMC: 7169750. DOI: 10.1111/tbed.12756. View

13.

Esona M, Geyer A, Banyai K, Page N, Aminu M, Armah G . Novel human rotavirus genotype G5P[7] from child with diarrhea, Cameroon. Emerg Infect Dis. 2009; 15(1):83-6. PMC: 2662634. DOI: 10.3201/eid1501.080899. View

14.

Settembre E, Chen J, Dormitzer P, Grigorieff N, Harrison S . Atomic model of an infectious rotavirus particle. EMBO J. 2010; 30(2):408-16. PMC: 3025467. DOI: 10.1038/emboj.2010.322. View

15.

Esona M, Roy S, Rungsrisuriyachai K, Sanchez J, Vasquez L, Gomez V . Characterization of a triple-recombinant, reassortant rotavirus strain from the Dominican Republic. J Gen Virol. 2016; 98(2):134-142. PMC: 5720362. DOI: 10.1099/jgv.0.000688. View

16.

He B, Yang F, Yang W, Zhang Y, Feng Y, Zhou J . Characterization of a novel G3P[3] rotavirus isolated from a lesser horseshoe bat: a distant relative of feline/canine rotaviruses. J Virol. 2013; 87(22):12357-66. PMC: 3807891. DOI: 10.1128/JVI.02013-13. View

17.

Banyai K, Kemenesi G, Budinski I, Foldes F, Zana B, Marton S . Candidate new rotavirus species in Schreiber's bats, Serbia. Infect Genet Evol. 2016; 48:19-26. PMC: 7106153. DOI: 10.1016/j.meegid.2016.12.002. View

18.

Masuda T, Nagai M, Yamasato H, Tsuchiaka S, Okazaki S, Katayama Y . Identification of novel bovine group A rotavirus G15P[14] strain from epizootic diarrhea of adult cows by de novo sequencing using a next-generation sequencer. Vet Microbiol. 2014; 171(1-2):66-73. PMC: 7127257. DOI: 10.1016/j.vetmic.2014.03.009. View

19.

Ghosh S, Alam M, Ahmed M, Talukdar R, Paul S, Kobayashi N . Complete genome constellation of a caprine group A rotavirus strain reveals common evolution with ruminant and human rotavirus strains. J Gen Virol. 2010; 91(Pt 9):2367-73. DOI: 10.1099/vir.0.022244-0. View

20.

Perez-Losada M, Arenas M, Galan J, Palero F, Gonzalez-Candelas F . Recombination in viruses: mechanisms, methods of study, and evolutionary consequences. Infect Genet Evol. 2014; 30:296-307. PMC: 7106159. DOI: 10.1016/j.meegid.2014.12.022. View