Identification of Novel Bovine Group A Rotavirus G15P[14] Strain from Epizootic Diarrhea of Adult Cows by De Novo Sequencing Using a Next-generation Sequencer

Overview

Journal Vet Microbiol

Specialties Microbiology
Veterinary Medicine

Date 2014 Apr 15

PMID 24725447

Citations 17

Authors

Tsuneyuki Masuda

Makoto Nagai

Hiroshi Yamasato

Shinobu Tsuchiaka

Sachiko Okazaki

Yukie Katayama

Mami Oba

Naomi Nishiura

Yukiko Sassa

Tsutomu Omatsu

Tetsuya Furuya

Satoshi Koyama

Junsuke Shirai

Koki Taniguchi

Yoshiki Fujii

Reiko Todaka

Kazuhiko Katayama

Tetsuya Mizutani

Affiliations

Soon will be listed here.

Abstract

There are few reports describing diarrhea of adult cattle caused by group A rotaviruses. Here, we report the identification of a novel bovine group A rotavirus from diarrhea of adult cows. A group A rotavirus was detected from an epizootic outbreak of diarrhea in adult cows with a decrease in milk production in Japan in 2013. The comprehensive genomic analyses from fecal samples by viral metagenomics using a next-generation sequencer revealed that it had an unreported genotype combination G15P[14]. The genome constellation of this strain, namely, RVA/Cow-wt/JPN/Tottori-SG/2013/G15P[14] was G15-P[14]-I2-R2-C2-M2-A3-N2-T6-E2-H3 representing VP7-VP4-VP6-VP1-VP2-VP3-NSP1-NSP2-NSP3-NSP4-NSP5, respectively. Each gene segment of Tottori-SG was most closely related to Japanese bovine group A rotaviruses suggesting that Tottori-SG might have derived from multiple reassortment events from group A rotavirus strains circulating among Japanese cattle. No other diarrhea pathogen of adult cattle was detected by routine diagnosis and metagenomics. Viral metagenomics, using a next-generation sequencer, is useful to characterize group A rotaviruses from fecal samples and offers unbiased comprehensive investigations of pathogen.

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References

Minami-Fukuda F, Nagai M, Takai H, Murakami T, Ozawa T, Tsuchiaka S . Detection of bovine group a rotavirus using rapid antigen detection kits, rt-PCR and next-generation DNA sequencing. J Vet Med Sci. 2013; 75(12):1651-5. PMC: 3942952. DOI: 10.1292/jvms.13-0265. View

Djikeng A, Halpin R, Kuzmickas R, DePasse J, Feldblyum J, Sengamalay N . Viral genome sequencing by random priming methods. BMC Genomics. 2008; 9:5. PMC: 2254600. DOI: 10.1186/1471-2164-9-5. View

Griffin D, Fletcher M, Levy M, Ching-Lee M, Nogami R, Edwards L . Outbreaks of adult gastroenteritis traced to a single genotype of rotavirus. J Infect Dis. 2002; 185(10):1502-5. DOI: 10.1086/340218. View

Ciarlet M, Hoffmann C, Lorusso E, Baselga R, Cafiero M, Banyai K . Genomic characterization of a novel group A lamb rotavirus isolated in Zaragoza, Spain. Virus Genes. 2008; 37(2):250-65. DOI: 10.1007/s11262-008-0257-6. View

Ghosh S, Samajdar S, Sinha M, Kobayashi N, Taniguchi K, Naik T . Molecular characterization of rare bovine group A rotavirus G15P[11] and G15P[21] strains from eastern India: identification of simian SA11-like VP6 genes in G15P[21] strains. Virus Genes. 2008; 37(2):241-9. DOI: 10.1007/s11262-008-0260-y. View

Ciarlet M, Estes M, Conner M . Comparative amino acid sequence analysis of the outer capsid protein VP4 from four lapine rotavirus strains reveals identity with genotype P[14] human rotaviruses. Arch Virol. 1997; 142(5):1059-69. DOI: 10.1007/s007050050142. View

Mlera L, ONeill H, Jere K, van Dijk A . Whole-genome consensus sequence analysis of a South African rotavirus SA11 sample reveals a mixed infection with two close derivatives of the SA11-H96 strain. Arch Virol. 2012; 158(5):1021-30. DOI: 10.1007/s00705-012-1559-5. View

Midgley S, Hjulsager C, Larsen L, Falkenhorst G, Bottiger B . Suspected zoonotic transmission of rotavirus group A in Danish adults. Epidemiol Infect. 2011; 140(6):1013-7. DOI: 10.1017/S0950268811001981. View

Matthijnssens J, Potgieter C, Ciarlet M, Parreno V, Martella V, Banyai K . Are human P[14] rotavirus strains the result of interspecies transmissions from sheep or other ungulates that belong to the mammalian order Artiodactyla?. J Virol. 2009; 83(7):2917-29. PMC: 2655590. DOI: 10.1128/JVI.02246-08. View

10.

Anderson E, Weber S . Rotavirus infection in adults. Lancet Infect Dis. 2004; 4(2):91-9. PMC: 7106507. DOI: 10.1016/S1473-3099(04)00928-4. View

11.

Cowley D, Donato C, Roczo-Farkas S, Kirkwood C . Novel G10P[14] rotavirus strain, northern territory, Australia. Emerg Infect Dis. 2013; 19(8):1324-7. PMC: 3739504. DOI: 10.3201/eid.1908.121653. View

12.

Tsunemitsu H, Jiang B, Saif L . Sequence comparison of the VP7 gene encoding the outer capsid glycoprotein among animal and human group C rotaviruses. Arch Virol. 1996; 141(3-4):705-13. DOI: 10.1007/BF01718328. View

13.

Hu L, Crawford S, Czako R, Cortes-Penfield N, Smith D, Le Pendu J . Cell attachment protein VP8* of a human rotavirus specifically interacts with A-type histo-blood group antigen. Nature. 2012; 485(7397):256-9. PMC: 3350622. DOI: 10.1038/nature10996. View

14.

Watanabe M, Nakagomi T, KOSHIMURA Y, Nakagomi O . Direct evidence for genome segment reassortment between concurrently-circulating human rotavirus strains. Arch Virol. 2001; 146(3):557-70. DOI: 10.1007/s007050170162. View

15.

Parreno V, Bok K, Fernandez F, Gomez J . Molecular characterization of the first isolation of rotavirus in guanacos (Lama guanicoe). Arch Virol. 2004; 149(12):2465-71. DOI: 10.1007/s00705-004-0371-2. View

16.

Chinsangaram J, Akita G, Osburn B . Detection of bovine group B rotaviruses in feces by polymerase chain reaction. J Vet Diagn Invest. 1994; 6(3):302-7. DOI: 10.1177/104063879400600304. View

17.

Gerna G, Sears J, Hoshino Y, Steele A, Nakagomi O, Sarasini A . Identification of a new VP4 serotype of human rotaviruses. Virology. 1994; 200(1):66-71. DOI: 10.1006/viro.1994.1163. View

18.

Abe M, Ito N, Morikawa S, Takasu M, Murase T, Kawashima T . Molecular epidemiology of rotaviruses among healthy calves in Japan: isolation of a novel bovine rotavirus bearing new P and G genotypes. Virus Res. 2009; 144(1-2):250-7. DOI: 10.1016/j.virusres.2009.05.005. View

19.

Matthijnssens J, Rahman M, Martella V, Xuelei Y, De Vos S, De Leener K . Full genomic analysis of human rotavirus strain B4106 and lapine rotavirus strain 30/96 provides evidence for interspecies transmission. J Virol. 2006; 80(8):3801-10. PMC: 1440464. DOI: 10.1128/JVI.80.8.3801-3810.2006. View

20.

Mphahlele M, Peenze I, Steele A . Rotavirus strains bearing the VP4P[14] genotype recovered from South African children with diarrhoea. Arch Virol. 1999; 144(5):1027-34. DOI: 10.1007/s007050050565. View