Cre-dependent Cas9-expressing Pigs Enable Efficient in Vivo Genome Editing

Overview

Journal Genome Res

Specialty Genetics

Date 2017 Nov 18

PMID 29146772

Citations 34

Authors

Kepin Wang

Qin Jin

Degong Ruan

Yi Yang

Qishuai Liu

Han Wu

Zhiwei Zhou

Zhen Ouyang

Zhaoming Liu

Yu Zhao

Bentian Zhao

Quanjun Zhang

Jiangyun Peng

Chengdan Lai

Nana Fan

Yanhui Liang

Ting Lan

Nan Li

Xiaoshan Wang

Xinlu Wang

Yong Fan

Pieter A Doevendans

Joost P G Sluijter

Pentao Liu

Xiaoping Li

Liangxue Lai

Affiliations

Soon will be listed here.

Abstract

Despite being time-consuming and costly, generating genome-edited pigs holds great promise for agricultural, biomedical, and pharmaceutical applications. To further facilitate genome editing in pigs, we report here establishment of a pig line with Cre-inducible Cas9 expression that allows a variety of ex vivo genome editing in fibroblast cells including single- and multigene modifications, chromosome rearrangements, and efficient in vivo genetic modifications. As a proof of principle, we were able to simultaneously inactivate five tumor suppressor genes (, , , , and ) and activate one oncogene (), achieved by delivering Cre recombinase and sgRNAs, which caused rapid lung tumor development. The efficient genome editing shown here demonstrates that these pigs can serve as a powerful tool for dissecting in vivo gene functions and biological processes in a temporal manner and for streamlining the production of genome-edited pigs for disease modeling.

Citing Articles

gene editing using primary cells derived from Cas9-expressing pigs.

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Functional integration of Cas9 gene into the genome of rhesus monkey: possibility of a new biomedical model?.

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Genome editing: An insight into disease resistance, production efficiency, and biomedical applications in livestock.

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