Biochanin A Induces Anticancer Effects in SK-Mel-28 Human Malignant Melanoma Cells Via Induction of Apoptosis, Inhibition of Cell Invasion and Modulation of NF-κB and MAPK Signaling Pathways

Overview

Journal Oncol Lett

Specialty Oncology

Date 2017 Nov 9

PMID 29113236

Citations 6

Authors

Peng Xiao

Bowen Zheng

Jiaming Sun

Jia Yang

Affiliations

Soon will be listed here.

Abstract

The present study aimed to investigate the antitumor activity of Biochanin A in SK-Mel-28 human malignant melanoma cells. An MTT assay was used to study the cytotoxic effects of Biochanin A. wound healing and invasion assays were used to investigate the effects on cell migration and invasion. Fluorescence microscopy using acridine orange/propidium iodide was used to study effects on cell morphology and apoptosis. Nuclear factor (NF)-κB and mitogen-activated protein kinase (MAPK) protein expression levels were determined by western blot analysis. The results indicated that Biochanin A significantly inhibited the growth of SK-Mel-28 cells in a dose and time dependent manner. Treatment of the cells with Biochanin A induced apoptosis in a dose dependent manner. Additionally, Biochanin A led to inhibition of cell migration and invasion in a dose-dependent manner and upregulated the expression of key proteins in the NF-κB and MAPK signaling pathways.

Citing Articles

Isoflavones: Promising Natural Agent for Cancer Prevention and Treatment.

Ul Hassan M, Shahbaz M, Imran M, Momal U, Naeem H, Mujtaba A Food Sci Nutr. 2025; 13(3):e70091.

PMID: 40078339 PMC: 11896816. DOI: 10.1002/fsn3.70091.

Biochanin A inhibits lung adenocarcinoma progression by targeting ZEB1.

Li J, Kou Y, Zhang X, Xiao X, Ou Y, Cao L Discov Oncol. 2022; 13(1):138.

PMID: 36512117 PMC: 9748019. DOI: 10.1007/s12672-022-00601-2.

Natural Small Molecules in Breast Cancer Treatment: Understandings from a Therapeutic Viewpoint.

Islam M, Islam F, Nafady M, Akter M, Mitra S, Das R Molecules. 2022; 27(7).

PMID: 35408561 PMC: 9000328. DOI: 10.3390/molecules27072165.

Inhibition of Melanoma Cell Migration and Invasion Targeting the Hypoxic Tumor Associated CAXII.

Giuntini G, Monaci S, Cau Y, Mori M, Naldini A, Carraro F Cancers (Basel). 2020; 12(10).

PMID: 33080820 PMC: 7602957. DOI: 10.3390/cancers12103018.

Anti-Melanoma Activities and Phytochemical Compositions of Fruit Extracts.

Jin S, Kim K, Kim J, Jang K, Hyun T Plants (Basel). 2020; 9(9).

PMID: 32825598 PMC: 7570188. DOI: 10.3390/plants9091076.

References

Sehdev V, Lai J, Bhushan A . Biochanin A Modulates Cell Viability, Invasion, and Growth Promoting Signaling Pathways in HER-2-Positive Breast Cancer Cells. J Oncol. 2010; 2009:121458. PMC: 2821773. DOI: 10.1155/2009/121458. View

Hodek P, Trefil P, Stiborova M . Flavonoids-potent and versatile biologically active compounds interacting with cytochromes P450. Chem Biol Interact. 2002; 139(1):1-21. DOI: 10.1016/s0009-2797(01)00285-x. View

Surh Y . Cancer chemoprevention with dietary phytochemicals. Nat Rev Cancer. 2003; 3(10):768-80. DOI: 10.1038/nrc1189. View

Comis R . DTIC (NSC-45388) in malignant melanoma: a perspective. Cancer Treat Rep. 1976; 60(2):165-76. View

Bhardwaj V, Tadinada S, Jain A, Sehdev V, Daniels C, Lai J . Biochanin A reduces pancreatic cancer survival and progression. Anticancer Drugs. 2013; 25(3):296-302. DOI: 10.1097/CAD.0000000000000044. View

Seo Y, Kim B, Chun S, Park Y, Kang K, Kwon T . Apoptotic effects of genistein, biochanin-A and apigenin on LNCaP and PC-3 cells by p21 through transcriptional inhibition of polo-like kinase-1. J Korean Med Sci. 2011; 26(11):1489-94. PMC: 3207053. DOI: 10.3346/jkms.2011.26.11.1489. View

Yang C, Landau J, Huang M, Newmark H . Inhibition of carcinogenesis by dietary polyphenolic compounds. Annu Rev Nutr. 2001; 21:381-406. DOI: 10.1146/annurev.nutr.21.1.381. View

Manthey J, Grohmann K, Guthrie N . Biological properties of citrus flavonoids pertaining to cancer and inflammation. Curr Med Chem. 2001; 8(2):135-53. DOI: 10.2174/0929867013373723. View

Senderowicz A . Novel small molecule cyclin-dependent kinases modulators in human clinical trials. Cancer Biol Ther. 2003; 2(4 Suppl 1):S84-95. View

10.

Soengas M, Lowe S . Apoptosis and melanoma chemoresistance. Oncogene. 2003; 22(20):3138-51. DOI: 10.1038/sj.onc.1206454. View

11.

McClay E, McClay M . Tamoxifen: is it useful in the treatment of patients with metastatic melanoma?. J Clin Oncol. 1994; 12(3):617-26. DOI: 10.1200/JCO.1994.12.3.617. View

12.

Medjakovic S, Jungbauer A . Red clover isoflavones biochanin A and formononetin are potent ligands of the human aryl hydrocarbon receptor. J Steroid Biochem Mol Biol. 2007; 108(1-2):171-7. DOI: 10.1016/j.jsbmb.2007.10.001. View

13.

Hussein M, Haemel A, Wood G . Apoptosis and melanoma: molecular mechanisms. J Pathol. 2003; 199(3):275-88. DOI: 10.1002/path.1300. View

14.

Galati G, Teng S, Moridani M, Chan T, OBrien P . Cancer chemoprevention and apoptosis mechanisms induced by dietary polyphenolics. Drug Metabol Drug Interact. 2001; 17(1-4):311-49. DOI: 10.1515/dmdi.2000.17.1-4.311. View

15.

Li X, Yang G, Li X, Zhang Y, Yang J, Chang J . Traditional Chinese medicine in cancer care: a review of controlled clinical studies published in chinese. PLoS One. 2013; 8(4):e60338. PMC: 3616129. DOI: 10.1371/journal.pone.0060338. View

16.

Park G, Kim D, Kim Y, Lee H, Kim C, Hur D . Silencing of galectin-3 represses osteosarcoma cell migration and invasion through inhibition of FAK/Src/Lyn activation and β-catenin expression and increases susceptibility to chemotherapeutic agents. Int J Oncol. 2014; 46(1):185-94. DOI: 10.3892/ijo.2014.2721. View