Anti-Melanoma Activities and Phytochemical Compositions of Fruit Extracts

Overview

Journal Plants (Basel)

Date 2020 Aug 23

PMID 32825598

Citations 9

Authors

Sora Jin

Kyeoung Cheol Kim

Ju-Sung Kim

Keum-Il Jang

Tae Kyung Hyun

Affiliations

Soon will be listed here.

Abstract

Hedl. (Rosaceae family) has a long history as a medicinal plant in East Asian countries. In this study, we evaluated the effect of fruit extracts prepared with different ethanol concentrations on anti-melanoma activity, and the extraction yield of phenolic compounds and flavonoids. Using the partitioned fractions from the EtOH extract, we found that the butanol fraction (BF) possessed strong cytotoxic activity against SK-MEL-2 cells (human melanoma cells) but not against HDFa cells (human dermal fibroblast adult cells). Additionally, BF-induced cell death was mediated by the inhibition of the mitogen-activated protein kinase/extracellular regulated kinase (MEK/ERK) signaling pathway, coupled with the upregulation of caspase-3 activity in SK-MEL-2 cells. Furthermore, HPLC analysis of polyphenolic compounds suggested that fruits contained several active compounds including chlorogenic acid, rutin, protocatechuic acid, and hydroxybenzoic acid, all of which are known to possess anti-cancer activities. Although this study has been carried out by cell-based approach, these results suggest that fruits contain promising anti-melanoma compounds.

Citing Articles

Fruit Extract Suppresses Lipopolysaccharide-Induced Neuroinflammation in BV-2 Microglia Cells via the MAPK and NF-B Signaling Pathways.

Kim Y, Jung J, Kim K Molecules. 2024; 29(23).

PMID: 39683751 PMC: 11643002. DOI: 10.3390/molecules29235592.

Antiviral activity of the water extract and ethanol extract of against influenza A virus .

Kim Y, Lee S, Kim C, Yoon S, Jeon S, Kweon M Heliyon. 2024; 10(20):e39049.

PMID: 39640637 PMC: 11620158. DOI: 10.1016/j.heliyon.2024.e39049.

Anticancer potential of hydroxycinnamic acids: mechanisms, bioavailability, and therapeutic applications.

Ceylan F, Gunal-Koroglu D, Saricaoglu B, Ozkan G, Capanoglu E, Calina D Naunyn Schmiedebergs Arch Pharmacol. 2024; 398(1):469-495.

PMID: 39212736 DOI: 10.1007/s00210-024-03396-x.

Phenolic acids and a static magnetic field change the expression of transforming growth factor β isoforms in amelanotic melanoma cells.

Kimsa-Dudek M, Synowiec-Wojtarowicz A, Krawczyk A Mol Biol Rep. 2023; 50(5):4207-4216.

PMID: 36899279 PMC: 10147755. DOI: 10.1007/s11033-023-08336-1.

In Vitro Antimelanoma Properties of Fractions.

Nisar R, Adhikary S, Ahmad S, Alam M Molecules. 2022; 27(19).

PMID: 36234866 PMC: 9571856. DOI: 10.3390/molecules27196329.

References

Hussein M, Haemel A, Wood G . Apoptosis and melanoma: molecular mechanisms. J Pathol. 2003; 199(3):275-88. DOI: 10.1002/path.1300. View

Afanasev I . Reactive oxygen species signaling in cancer: comparison with aging. Aging Dis. 2012; 2(3):219-30. PMC: 3295056. View

Sohn E, Kang D, Choi D, Lee A, Mun Y, Woo W . Effect of methanol extract of Sorbus cortex in a rat model of L-NAME-induced atherosclerosis. Biol Pharm Bull. 2005; 28(7):1239-43. DOI: 10.1248/bpb.28.1239. View

ODonovan N, Crown J, Stunell H, Hill A, McDermott E, OHiggins N . Caspase 3 in breast cancer. Clin Cancer Res. 2003; 9(2):738-42. View

Do Q, Angkawijaya A, Tran-Nguyen P, Huynh L, Soetaredjo F, Ismadji S . Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. J Food Drug Anal. 2017; 22(3):296-302. PMC: 9354875. DOI: 10.1016/j.jfda.2013.11.001. View

Cheung E, Slack R . Emerging role for ERK as a key regulator of neuronal apoptosis. Sci STKE. 2004; 2004(251):PE45. DOI: 10.1126/stke.2512004pe45. View

Yin M, Kang D, Choi D, Kwon T, Lee H . Screening of vasorelaxant activity of some medicinal plants used in Oriental medicines. J Ethnopharmacol. 2005; 99(1):113-7. DOI: 10.1016/j.jep.2005.02.013. View

Caparica R, Julio A, Machado Araujo M, Baby A, Fonte P, Costa J . Anticancer Activity of Rutin and Its Combination with Ionic Liquids on Renal Cells. Biomolecules. 2020; 10(2). PMC: 7072522. DOI: 10.3390/biom10020233. View

Libra M, Malaponte G, Navolanic P, Gangemi P, Bevelacqua V, Proietti L . Analysis of BRAF mutation in primary and metastatic melanoma. Cell Cycle. 2005; 4(10):1382-4. DOI: 10.4161/cc.4.10.2026. View

10.

Vikey A, Vikey D . Primary malignant melanoma, of head and neck: a comprehensive review of literature. Oral Oncol. 2012; 48(5):399-403. DOI: 10.1016/j.oraloncology.2011.12.014. View

11.

Guo Y, Pan W, Liu S, Shen Z, Xu Y, Hu L . ERK/MAPK signalling pathway and tumorigenesis. Exp Ther Med. 2020; 19(3):1997-2007. PMC: 7027163. DOI: 10.3892/etm.2020.8454. View

12.

Lee S, Lee H, Lee I, Im H . The pharmacological potential of Sorbus commixta cortex on blood alcohol concentration and hepatic lipid peroxidation in acute alcohol-treated rats. J Pharm Pharmacol. 2006; 58(5):685-93. DOI: 10.1211/jpp.58.5.0014. View

13.

Liu D, Qiu X, Xiong X, Chen X, Pan F . Current updates on the role of reactive oxygen species in bladder cancer pathogenesis and therapeutics. Clin Transl Oncol. 2020; 22(10):1687-1697. PMC: 7423792. DOI: 10.1007/s12094-020-02330-w. View

14.

Yan Y, Li J, Han J, Hou N, Song Y, Dong L . Chlorogenic acid enhances the effects of 5-fluorouracil in human hepatocellular carcinoma cells through the inhibition of extracellular signal-regulated kinases. Anticancer Drugs. 2015; 26(5):540-6. PMC: 4415958. DOI: 10.1097/CAD.0000000000000218. View

15.

Srivastava V, Negi A, Kumar J, Gupta M, Khanuja S . Plant-based anticancer molecules: a chemical and biological profile of some important leads. Bioorg Med Chem. 2005; 13(21):5892-908. DOI: 10.1016/j.bmc.2005.05.066. View

16.

Naveed M, Hejazi V, Abbas M, Kamboh A, Khan G, Shumzaid M . Chlorogenic acid (CGA): A pharmacological review and call for further research. Biomed Pharmacother. 2017; 97:67-74. DOI: 10.1016/j.biopha.2017.10.064. View

17.

Miller K, Siegel R, Lin C, Mariotto A, Kramer J, Rowland J . Cancer treatment and survivorship statistics, 2016. CA Cancer J Clin. 2016; 66(4):271-89. DOI: 10.3322/caac.21349. View

18.

Gao X, Schottker B . Reduction-oxidation pathways involved in cancer development: a systematic review of literature reviews. Oncotarget. 2017; 8(31):51888-51906. PMC: 5584299. DOI: 10.18632/oncotarget.17128. View

19.

Yu T, Lee Y, Jang H, Kim A, Hong S, Woong Kim T . Anti-inflammatory activity of Sorbus commixta water extract and its molecular inhibitory mechanism. J Ethnopharmacol. 2011; 134(2):493-500. DOI: 10.1016/j.jep.2010.12.032. View

20.

Hadi S, Asad S, Singh S, Ahmad A . Putative mechanism for anticancer and apoptosis-inducing properties of plant-derived polyphenolic compounds. IUBMB Life. 2001; 50(3):167-71. DOI: 10.1080/152165400300001471. View