Functional Characterization of a Hydroxyacid/Alcohol Hydroxycinnamoyl Transferase Produced by the Liverwort Marchantia Emarginata

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2017 Nov 1

PMID 29088080

Citations 2

Authors

Ping-Ping Wang

Hui Liu

Shuai Gao

Ai-Xia Cheng

Affiliations

Soon will be listed here.

Abstract

The aerial organs of most terrestrial plants are covered by a hydrophobic protective cuticle. The main constituent of the cuticle is the lipid polyester cutin, which is composed of aliphatic and aromatic domains. The aliphatic component is a polyester between fatty acid/alcohol and hydroxycinnamoyl acid. The BAHD/HxxxD family enzymes are central to the synthesis of these polyesters. The nature of this class of enzymes in bryophytes has not been explored to date. Here, a gene encoding a fatty ω-hydroxyacid/fatty alcohol hydroxycinnamoyl transferase (HFT) has been isolated from the liverwort and has been functionally characterized. Experiments based on recombinant protein showed that the enzyme uses ω-hydroxy fatty acids or primary alcohols as its acyl acceptor and various hydroxycinnamoyl-CoAs-preferentially feruloyl-CoA and caffeoyl-CoA-as acyl donors at least in vitro. The transient expression of a fusion transgene in the leaf demonstrated that MeHFT is directed to the cytoplasm, suggesting that the feruloylation of cutin monomers takes place there.

Citing Articles

Genome-Wide Identification and Expression Pattern Analysis of BAHD Acyltransferase Family in .

Xu D, Wang Z, Zhuang W, Zhang F, Xie Y, Wang T Int J Mol Sci. 2024; 25(7).

PMID: 38612586 PMC: 11011543. DOI: 10.3390/ijms25073777.

Family characteristics, phylogenetic reconstruction, and potential applications of the plant BAHD acyltransferase family.

Xu D, Wang Z, Zhuang W, Wang T, Xie Y Front Plant Sci. 2023; 14:1218914.

PMID: 37868312 PMC: 10585174. DOI: 10.3389/fpls.2023.1218914.

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