Restriction Fragment Polymorphism in the Sex-determining Region of the Y Chromosomal DNA of European Wild Mice

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1988 Jun 1

PMID 2901661

Citations 7

Authors

L Singh

H Winking

K W Jones

A Gropp

Affiliations

Soon will be listed here.

Abstract

Using 32P-labeled probe consisting mainly of (GATA)n we have shown that a male specific Alu1 DNA blot pattern which defines the Y chromosome sex-determining locus in inbred mice is highly polymorphic in wild mice, indicating substantial sequence evolution in this region under field conditions. In all cases examined by in situ hybridization, the region concerned is paracentromeric. In contrast, the blot pattern of another probe (M 34) which detects repeated sequences specific to the mouse Y chromosome but outside the sex-determining locus, remains constant between different isolates.

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References

Bishop C, Boursot P, Baron B, Bonhomme F, Hatat D . Most classical Mus musculus domesticus laboratory mouse strains carry a Mus musculus musculus Y chromosome. Nature. 1985; 315(6014):70-2. DOI: 10.1038/315070a0. View

Lamar E, Palmer E . Y-encoded, species-specific DNA in mice: evidence that the Y chromosome exists in two polymorphic forms in inbred strains. Cell. 1984; 37(1):171-7. DOI: 10.1016/0092-8674(84)90312-x. View

Cooke H, Brown W, Rappold G . Hypervariable telomeric sequences from the human sex chromosomes are pseudoautosomal. Nature. 1985; 317(6039):687-92. DOI: 10.1038/317687a0. View

Singh L, Phillips C, Jones K . The conserved nucleotide sequences of Bkm, which define Sxr in the mouse, are transcribed. Cell. 1984; 36(1):111-20. DOI: 10.1016/0092-8674(84)90079-5. View

Singh L, Jones K . Bkm sequences are polymorphic in humans and are clustered in pericentric regions of various acrocentric chromosomes including the Y. Hum Genet. 1986; 73(4):304-8. DOI: 10.1007/BF00279091. View

Singh L, Purdom I, Jones K . Conserved sex-chromosome-associated nucleotide sequences in eukaryotes. Cold Spring Harb Symp Quant Biol. 1981; 45 Pt 2:805-14. DOI: 10.1101/sqb.1981.045.01.099. View

Bonhomme F, Catalan J, Britton-Davidian J, Chapman V, Moriwaki K, Nevo E . Biochemical diversity and evolution in the genus Mus. Biochem Genet. 1984; 22(3-4):275-303. DOI: 10.1007/BF00484229. View

Traut W . Hypervariable Bkm DNA Loci in a Moth, Ephestia kuehniella : Does Transposition Cause Restriction Fragment Length Polymorphism?. Genetics. 1987; 115(3):493-8. PMC: 1216351. DOI: 10.1093/genetics/115.3.493. View

Washburn L, Eicher E . Sex reversal in XY mice caused by dominant mutation on chromosome 17. Nature. 1983; 303(5915):338-40. DOI: 10.1038/303338a0. View

10.

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

11.

Melton D, Krieg P, Rebagliati M, Maniatis T, Zinn K, Green M . Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res. 1984; 12(18):7035-56. PMC: 320141. DOI: 10.1093/nar/12.18.7035. View

12.

Hu N, Messing J . The making of strand-specific M13 probes. Gene. 1982; 17(3):271-7. DOI: 10.1016/0378-1119(82)90143-3. View

13.

Jones K, Singh L . Conserved repeated DNA sequences in vertebrate sex chromosomes. Hum Genet. 1981; 58(1):46-53. DOI: 10.1007/BF00284148. View

14.

Singh L, Jones K . The use of heparin as a simple cost-effective means of controlling background in nucleic acid hybridization procedures. Nucleic Acids Res. 1984; 12(14):5627-38. PMC: 320019. DOI: 10.1093/nar/12.14.5627. View

15.

Singh L, Jones K . Sex reversal in the mouse (Mus musculus) is caused by a recurrent nonreciprocal crossover involving the x and an aberrant y chromosome. Cell. 1982; 28(2):205-16. DOI: 10.1016/0092-8674(82)90338-5. View

16.

Forejt J, Ivanyi P . Genetic studies on male sterility of hybrids between laboratory and wild mice (Mus musculus L.). Genet Res. 1974; 24(2):189-206. DOI: 10.1017/s0016672300015214. View

17.

Erickson R . Regional localization of sex-specific Bkm-related sequences on proximal chromosome 17 of mice. Nature. 1984; 310(5978):579-81. DOI: 10.1038/310579a0. View

18.

Wahl G, Stern M, Stark G . Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979; 76(8):3683-7. PMC: 383897. DOI: 10.1073/pnas.76.8.3683. View

19.

Livak K . Organization and mapping of a sequence on the Drosophila melanogaster X and Y chromosomes that is transcribed during spermatogenesis. Genetics. 1984; 107(4):611-34. PMC: 1202380. DOI: 10.1093/genetics/107.4.611. View

20.

Bittner M, Kupferer P, Morris C . Electrophoretic transfer of proteins and nucleic acids from slab gels to diazobenzyloxymethyl cellulose or nitrocellulose sheets. Anal Biochem. 1980; 102(2):459-71. DOI: 10.1016/0003-2697(80)90182-7. View