Genetic Analysis of Porcine Circovirus Type 2 from Pigs Affected with PMWS in Chile Reveals Intergenotypic Recombination

Overview

Journal Virol J

Publisher Biomed Central

Specialty Microbiology

Date 2017 Oct 6

PMID 28978346

Citations 7

Authors

Victor Neira

Natalia Ramos

Rodrigo Tapia

Juan Arbiza

Andronico Neira-Carrillo

Manuel Quezada

Alvaro Ruiz

Sergio A Bucarey

Affiliations

Soon will be listed here.

Abstract

Background: Porcine circovirus type 2 (PCV2) is a very small, non-enveloped and icosahedral virus, with circular single stranded DNA genome. This virus is the most ubiquitous and persistent pathogen currently affecting the swine industry worldwide. PCV2 has been implicated as the major causative agent of postweaning multisystemic wasting syndrome (PMWS), a disease which is characterized by severe immunosuppressive effects in the porcine host. Worldwide PCV2 isolates have been classified into four different genotypes, PCV2a, PCV2b, PCV2c and PCVd. The goal of this work was to conduct the first phylogenetic analysis of PCV2 in Chile.

Methods: PCV2 partial ORF2 sequences (462 nt) obtained from 29 clinical cases of PMWS in 22 Chilean intensive swine farms, covering over the 90% of the local pork-production, were analyzed.

Results: 14% and 52% of sequences belonged to the genotypes PCV2a and PCV2b, respectively. Surprisingly, 34% of sequences were PCV2a/PCV2d recombinant viruses.

Conclusions: Our findings suggested that a novel cluster of Chilean sequences emerged resulting from intergenotypic recombination between PCV2a and PCV2d.

Citing Articles

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Update of Genetic Diversity of Porcine Circovirus Type 2 in Chile Evidences the Emergence of PCV2d Genotype.

Ariyama N, Aguero B, Valdes V, Berrios F, Bucarey S, Mor S Front Vet Sci. 2022; 8:789491.

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Mena J, Ariyama N, Navarro C, Quezada M, Brevis C, Rojas D Transbound Emerg Dis. 2021; 68(6):3174-3179.

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