The Cytosolic 5'-nucleotidase CN-II Lowers the Adaptability to Glucose Deprivation in Human Breast Cancer Cells

Overview

Journal Oncotarget

Specialty Oncology

Date 2017 Oct 6

PMID 28978040

Citations 8

Authors

Gabriel Bricard

Octavia Cadassou

Laure-Estelle Cassagnes

Emeline Cros-Perrial

Lea Payen-Gay

Jean-Yves Puy

Isabelle Lefebvre-Tournier

Maria Grazia Tozzi

Charles Dumontet

Lars Petter Jordheim

Affiliations

Soon will be listed here.

Abstract

The cytosolic 5'-nucleotidase cN-II is a highly conserved enzyme implicated in nucleotide metabolism. Based on recent observations suggesting additional roles not directly associated to its enzymatic activity, we studied human cancer cell models with basal or decreased cN-II expression. We developed cancer cells with stable inhibition of cN-II expression by transfection of shRNA-coding plasmids, and studied their biology. We show that human breast cancer cells MDA-MB-231 with decreased cN-II expression better adapt to the disappearance of glucose in growth medium under normoxic conditions than cells with a baseline expression level. This is associated with enhanced growth and a lower content of ROS in cells cultivated in absence of glucose due to more efficient mechanisms of elimination of ROS. Conversely, cells with low cN-II expression are more sensitive to glucose deprivation in hypoxic conditions. Overall, our results show that cN-II regulates the cellular response to glucose deprivation through a mechanism related to ROS metabolism and defence.

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