Significant Hydrolysis of Wheat Gliadin by Bacillus Tequilensis (10bT/HQ223107): a Pilot Study

Overview

Journal Probiotics Antimicrob Proteins

Publisher Springer

Specialties Infectious Diseases
Microbiology
Nutritional Sciences
Pharmacology

Date 2017 Sep 27

PMID 28948492

Citations 5

Authors

Sandip K Wagh

Praful P Gadge

Manohar V Padul

Affiliations

Soon will be listed here.

Abstract

Peptidase therapy is suggested to be effective to minimize gliadin toxicity in celiac disease (CD). Hence, present study deals with gliadin-hydrolysing peptidases. The efficient peptidase from the Bacillus tequilensis was purified using ammonium sulfate fractionation and preparative electrophoresis. Analysis of in-solution and in-gel hydrolysis of gliadin using one and two-dimensional SDS-PAGE revealed nearly complete hydrolysis of gliadin peptides after 180 min of incubation with B. tequilensis protease. Purified peptidase was found to be stable at acidic (pH 3.5) to neutral (pH 7.2) pH range. The molecular mass and isoelectric point of the peptidase were observed around 29 kDa and 5.2, respectively. The internal protein sequence obtained through mass spectrometric analysis suggested that peptidase might belong to peptidase S9 family known for prolyl-specific peptidases. This study recommends the possible applicability of this peptidase for elimination of immunotoxic gliadin peptides and may prove useful in CD treatment.

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References

Duar R, Clark K, Patil P, Hernandez C, Bruning S, Burkey T . Identification and characterization of intestinal lactobacilli strains capable of degrading immunotoxic peptides present in gluten. J Appl Microbiol. 2014; 118(2):515-27. DOI: 10.1111/jam.12687. View

Maki M, Mustalahti K, Kokkonen J, Kulmala P, Haapalahti M, Karttunen T . Prevalence of Celiac disease among children in Finland. N Engl J Med. 2003; 348(25):2517-24. DOI: 10.1056/NEJMoa021687. View

Helmerhorst E, Zamakhchari M, Schuppan D, Oppenheim F . Discovery of a novel and rich source of gluten-degrading microbial enzymes in the oral cavity. PLoS One. 2010; 5(10):e13264. PMC: 2952584. DOI: 10.1371/journal.pone.0013264. View

Stepniak D, Spaenij-Dekking L, Mitea C, Moester M, de Ru A, Baak-Pablo R . Highly efficient gluten degradation with a newly identified prolyl endoprotease: implications for celiac disease. Am J Physiol Gastrointest Liver Physiol. 2006; 291(4):G621-9. DOI: 10.1152/ajpgi.00034.2006. View

Matysiak-Budnik T, Candalh C, Cellier C, Dugave C, Namane A, Vidal-Martinez T . Limited efficiency of prolyl-endopeptidase in the detoxification of gliadin peptides in celiac disease. Gastroenterology. 2005; 129(3):786-96. DOI: 10.1053/j.gastro.2005.06.016. View

Saitou N, Nei M . The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987; 4(4):406-25. DOI: 10.1093/oxfordjournals.molbev.a040454. View

Severance E, Yolken R, Eaton W . Autoimmune diseases, gastrointestinal disorders and the microbiome in schizophrenia: more than a gut feeling. Schizophr Res. 2014; 176(1):23-35. PMC: 4294997. DOI: 10.1016/j.schres.2014.06.027. View

Gadge P, Wagh S, Shaikh F, Tak R, Padul M, Kachole M . A bifunctional α-amylase/trypsin inhibitor from pigeonpea seeds: Purification, biochemical characterization and its bio-efficacy against Helicoverpa armigera. Pestic Biochem Physiol. 2015; 125:17-25. DOI: 10.1016/j.pestbp.2015.06.007. View

Roxas M . The role of enzyme supplementation in digestive disorders. Altern Med Rev. 2009; 13(4):307-14. View

10.

Horikoshi K . Alkaliphiles: some applications of their products for biotechnology. Microbiol Mol Biol Rev. 1999; 63(4):735-50, table of contents. PMC: 98975. DOI: 10.1128/MMBR.63.4.735-750.1999. View

11.

Sollid L . Coeliac disease: dissecting a complex inflammatory disorder. Nat Rev Immunol. 2002; 2(9):647-55. DOI: 10.1038/nri885. View

12.

Anisha A, Anandham R, Kwon S, Gandhi P, Gopal N . Evaluation of Bacillus spp. as dough starters for Adhirasam - A traditional rice based fermented food of Southern India. Braz J Microbiol. 2015; 46(4):1183-91. PMC: 4704633. DOI: 10.1590/S1517-838246420140409. View

13.

Cunningham D, OConnor B . Proline specific peptidases. Biochim Biophys Acta. 1998; 1343(2):160-86. DOI: 10.1016/s0167-4838(97)00134-9. View

14.

Green P, Lebwohl B, Greywoode R . Celiac disease. J Allergy Clin Immunol. 2015; 135(5):1099-106. DOI: 10.1016/j.jaci.2015.01.044. View

15.

Fernandez-Feo M, Wei G, Blumenkranz G, Dewhirst F, Schuppan D, Oppenheim F . The cultivable human oral gluten-degrading microbiome and its potential implications in coeliac disease and gluten sensitivity. Clin Microbiol Infect. 2013; 19(9):E386-94. PMC: 3749263. DOI: 10.1111/1469-0691.12249. View

16.

Koning F . Celiac disease: quantity matters. Semin Immunopathol. 2012; 34(4):541-9. PMC: 3410019. DOI: 10.1007/s00281-012-0321-0. View

17.

Di Sabatino A, Vanoli A, Giuffrida P, Luinetti O, Solcia E, Corazza G . The function of tissue transglutaminase in celiac disease. Autoimmun Rev. 2012; 11(10):746-53. DOI: 10.1016/j.autrev.2012.01.007. View

18.

Constantin C, Huber W, Granditsch G, Weghofer M, Valenta R . Different profiles of wheat antigens are recognised by patients suffering from coeliac disease and IgE-mediated food allergy. Int Arch Allergy Immunol. 2005; 138(3):257-66. DOI: 10.1159/000088727. View

19.

Schuppan D, Junker Y, Barisani D . Celiac disease: from pathogenesis to novel therapies. Gastroenterology. 2009; 137(6):1912-33. DOI: 10.1053/j.gastro.2009.09.008. View

20.

Fulop V, Bocskei Z, Polgar L . Prolyl oligopeptidase: an unusual beta-propeller domain regulates proteolysis. Cell. 1998; 94(2):161-70. DOI: 10.1016/s0092-8674(00)81416-6. View