Construction of Human Induced Pluripotent Stem Cell-derived Oriented Bone Matrix Microstructure by Using in Vitro Engineered Anisotropic Culture Model

Overview

Journal J Biomed Mater Res A

Specialty Biomedical Engineering

Date 2017 Sep 19

PMID 28921822

Citations 12

Authors

Ryosuke Ozasa

Aira Matsugaki

Yoshihiro Isobe

Taro Saku

Hui-Suk Yun

Takayoshi Nakano

Affiliations

Soon will be listed here.

Abstract

Bone tissue has anisotropic microstructure based on collagen/biological apatite orientation, which plays essential roles in the mechanical and biological functions of bone. However, obtaining an appropriate anisotropic microstructure during the bone regeneration process remains a great challenging. A powerful strategy for the control of both differentiation and structural development of newly-formed bone is required in bone tissue engineering, in order to realize functional bone tissue regeneration. In this study, we developed a novel anisotropic culture model by combining human induced pluripotent stem cells (hiPSCs) and artificially-controlled oriented collagen scaffold. The oriented collagen scaffold allowed hiPSCs-derived osteoblast alignment and further construction of anisotropic bone matrix which mimics the bone tissue microstructure. To the best of our knowledge, this is the first report showing the construction of bone mimetic anisotropic bone matrix microstructure from hiPSCs. Moreover, we demonstrated for the first time that the hiPSCs-derived osteoblasts possess a high level of intact functionality to regulate cell alignment. © 2017 The Authors Journal of Biomedical Materials Research Part A Published by Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 360-369, 2018.

Citing Articles

Assembling the Puzzle Pieces. Insights for in Vitro Bone Remodeling.

Krasnova O, Neganova I Stem Cell Rev Rep. 2023; 19(6):1635-1658.

PMID: 37204634 DOI: 10.1007/s12015-023-10558-6.

Bone quality assessment of osteogenic cell cultures by Raman microscopy.

Mandair G, Steenhuis P, Ignelzi Jr M, Morris M J Raman Spectrosc. 2023; 50(3):360-370.

PMID: 37035410 PMC: 10081538. DOI: 10.1002/jrs.5521.

Preparation and performance of random- and oriented-fiber membranes with core-shell structures coaxial electrospinning.

Li Y, Jin D, Fan Y, Zhang K, Yang T, Zou C Front Bioeng Biotechnol. 2023; 10:1114034.

PMID: 36698642 PMC: 9868300. DOI: 10.3389/fbioe.2022.1114034.

Periodontal Tissue as a Biomaterial for Hard-Tissue Regeneration following Gene Transfer.

Kawai M, Ozasa R, Ishimoto T, Nakano T, Yamamoto H, Kashiwagi M Materials (Basel). 2022; 15(3).

PMID: 35160948 PMC: 8840059. DOI: 10.3390/ma15030993.

Superior Alignment of Human iPSC-Osteoblasts Associated with Focal Adhesion Formation Stimulated by Oriented Collagen Scaffold.

Ozasa R, Matsugaki A, Matsuzaka T, Ishimoto T, Yun H, Nakano T Int J Mol Sci. 2021; 22(12).

PMID: 34207766 PMC: 8228163. DOI: 10.3390/ijms22126232.

References

Lieber R, Friden J . Functional and clinical significance of skeletal muscle architecture. Muscle Nerve. 2000; 23(11):1647-66. DOI: 10.1002/1097-4598(200011)23:11<1647::aid-mus1>3.0.co;2-m. View

Ozasa R, Matsugaki A, Isobe Y, Saku T, Yun H, Nakano T . Construction of human induced pluripotent stem cell-derived oriented bone matrix microstructure by using in vitro engineered anisotropic culture model. J Biomed Mater Res A. 2017; 106(2):360-369. PMC: 5765486. DOI: 10.1002/jbm.a.36238. View

Matsugaki A, Aramoto G, Nakano T . The alignment of MC3T3-E1 osteoblasts on steps of slip traces introduced by dislocation motion. Biomaterials. 2012; 33(30):7327-35. DOI: 10.1016/j.biomaterials.2012.06.022. View

Taubenberger A, Woodruff M, Bai H, Muller D, Hutmacher D . The effect of unlocking RGD-motifs in collagen I on pre-osteoblast adhesion and differentiation. Biomaterials. 2010; 31(10):2827-35. DOI: 10.1016/j.biomaterials.2009.12.051. View

Kannus P . Structure of the tendon connective tissue. Scand J Med Sci Sports. 2000; 10(6):312-20. DOI: 10.1034/j.1600-0838.2000.010006312.x. View

Nakano T, Kaibara K, Tabata Y, Nagata N, Enomoto S, Marukawa E . Unique alignment and texture of biological apatite crystallites in typical calcified tissues analyzed by microbeam X-ray diffractometer system. Bone. 2002; 31(4):479-87. DOI: 10.1016/s8756-3282(02)00850-5. View

Yamazaki C, Kadoya Y, Hozumi K, Okano-Kosugi H, Asada S, Kitagawa K . A collagen-mimetic triple helical supramolecule that evokes integrin-dependent cell responses. Biomaterials. 2009; 31(7):1925-34. DOI: 10.1016/j.biomaterials.2009.10.014. View

Brown S, Tong W, Krebsbach P . The derivation of mesenchymal stem cells from human embryonic stem cells. Cells Tissues Organs. 2008; 189(1-4):256-60. PMC: 2690958. DOI: 10.1159/000151746. View

Murphy S, Atala A . Organ engineering--combining stem cells, biomaterials, and bioreactors to produce bioengineered organs for transplantation. Bioessays. 2012; 35(3):163-72. DOI: 10.1002/bies.201200062. View

10.

Romanov Y, Svintsitskaya V, Smirnov V . Searching for alternative sources of postnatal human mesenchymal stem cells: candidate MSC-like cells from umbilical cord. Stem Cells. 2003; 21(1):105-10. DOI: 10.1634/stemcells.21-1-105. View

11.

Ardeshirylajimi A, Hosseinkhani S, Parivar K, Yaghmaie P, Soleimani M . Nanofiber-based polyethersulfone scaffold and efficient differentiation of human induced pluripotent stem cells into osteoblastic lineage. Mol Biol Rep. 2013; 40(7):4287-94. DOI: 10.1007/s11033-013-2515-5. View

12.

Docheva D, Popov C, Alberton P, Aszodi A . Integrin signaling in skeletal development and function. Birth Defects Res C Embryo Today. 2014; 102(1):13-36. DOI: 10.1002/bdrc.21059. View

13.

Yang X, Bhatnagar R, Li S, Oreffo R . Biomimetic collagen scaffolds for human bone cell growth and differentiation. Tissue Eng. 2004; 10(7-8):1148-59. DOI: 10.1089/ten.2004.10.1148. View

14.

Nakano T, Kaibara K, Ishimoto T, Tabata Y, Umakoshi Y . Biological apatite (BAp) crystallographic orientation and texture as a new index for assessing the microstructure and function of bone regenerated by tissue engineering. Bone. 2012; 51(4):741-7. DOI: 10.1016/j.bone.2012.07.003. View

15.

Horiguchi A, Yazaki K, Aoyagi M, Ohnuki Y, Kurosawa H . Effective Rho-associated protein kinase inhibitor treatment to dissociate human iPS cells for suspension culture to form embryoid body-like cell aggregates. J Biosci Bioeng. 2014; 118(5):588-92. DOI: 10.1016/j.jbiosc.2014.04.008. View

16.

Takahashi K, Yamanaka S . Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 2006; 126(4):663-76. DOI: 10.1016/j.cell.2006.07.024. View

17.

Gasser T, Ogden R, Holzapfel G . Hyperelastic modelling of arterial layers with distributed collagen fibre orientations. J R Soc Interface. 2006; 3(6):15-35. PMC: 1618483. DOI: 10.1098/rsif.2005.0073. View

18.

Matsugaki A, Isobe Y, Saku T, Nakano T . Quantitative regulation of bone-mimetic, oriented collagen/apatite matrix structure depends on the degree of osteoblast alignment on oriented collagen substrates. J Biomed Mater Res A. 2014; 103(2):489-99. DOI: 10.1002/jbm.a.35189. View

19.

Savla J, Nelson B, Perry C, Adler E . Induced pluripotent stem cells for the study of cardiovascular disease. J Am Coll Cardiol. 2014; 64(5):512-9. DOI: 10.1016/j.jacc.2014.05.038. View

20.

Sekita A, Matsugaki A, Ishimoto T, Nakano T . Synchronous disruption of anisotropic arrangement of the osteocyte network and collagen/apatite in melanoma bone metastasis. J Struct Biol. 2016; 197(3):260-270. DOI: 10.1016/j.jsb.2016.12.003. View