Diversity of the Mouse T Cell Receptor C Gamma 1 Gene: Structural Analysis in C57BL/Ka

Overview

Journal Immunogenetics

Specialties Allergy & Immunology
Genetics

Date 1988 Jan 1

PMID 2891611

Citations 2

Authors

G A Rathbun

W Born

W A Kuziel

P W Tucker

Affiliations

Soon will be listed here.

Abstract

We have isolated an unusual T cell receptor gamma chain cDNA clone (gamma 7.1) from a library made from RNA derived from adult thymus of C57BL/Ka mice. This cDNA clone corresponds to the appropriately processed C gamma 1 constant region exons preceded by 1.5 kb of J-C gamma 1 intron. The gamma 7.1 coding region is extremely homologous to the C gamma 1 gene of BALB/c mice, differing at the protein level by a single deletion (alanine 139) and a single substitution. This latter change eliminates the sole N-linked sugar attachment site, providing a basis for strain-specific glycosylation patterns. The J-C gamma 1 intronic region contains two DNA segments (termed psi J gamma 1 and psi J gamma 2) that are highly reminiscent of joining (J) segments; both have potentially functional recombination and donor splice sequences flanking an open reading frame. Northern analysis suggests that gamma 7.1 may be derived from a large, variable region-containing precursor.

Citing Articles

New T-cell receptor gamma haplotypes in wild mice and evidence for limited Tcrg-V gene polymorphism.

Roger T, Pepin L, Jouvin-Marche E, Cazenave P, Seman M Immunogenetics. 1993; 37(3):161-9.

PMID: 8420824 DOI: 10.1007/BF00191880.

Polymorphism of Tcrb and Tcrg genes in Biozzi mice: segregation analysis of a new Tcrg haplotype with antibody responsiveness.

Vidard L, Roger T, Pham G, Couderc J, BOUTHILLIER Y, Mevel J Immunogenetics. 1990; 32(1):27-33.

PMID: 1973682 DOI: 10.1007/BF01787325.

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