Synthesis and Multiple Incorporations of 2'-O-Methyl-5-hydroxymethylcytidine, 5-Hydroxymethylcytidine and 5-Formylcytidine Monomers into RNA Oligonucleotides

Overview

Journal Chembiochem

Specialty Biochemistry

Date 2017 Sep 14

PMID 28901692

Citations 5

Authors

Arun A Tanpure

Shankar Balasubramanian

Affiliations

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Abstract

The synthesis of 2'-O-methyl-5-hydroxymethylcytidine (hm Cm), 5-hydroxymethylcytidine (hm C) and 5-formylcytidine (f C) phosphoramidite monomers has been developed. Optimisation of mild post-synthetic deprotection conditions enabled the synthesis of RNA containing all four naturally occurring cytosine modifications (hm Cm, hm C, f C plus 5-methylcytosine). Given the considerable interest in RNA modifications and epitranscriptomics, the availability of synthetic monomers and RNAs containing these modifications will be valuable for elucidating their biological function(s).

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