Splatter: Simulation of Single-cell RNA Sequencing Data

Overview

Journal Genome Biol

Specialties Biology
Genetics

Date 2017 Sep 14

PMID 28899397

Citations 360

Authors

Luke Zappia

Belinda Phipson

Alicia Oshlack

Affiliations

Soon will be listed here.

Abstract

As single-cell RNA sequencing (scRNA-seq) technologies have rapidly developed, so have analysis methods. Many methods have been tested, developed, and validated using simulated datasets. Unfortunately, current simulations are often poorly documented, their similarity to real data is not demonstrated, or reproducible code is not available. Here, we present the Splatter Bioconductor package for simple, reproducible, and well-documented simulation of scRNA-seq data. Splatter provides an interface to multiple simulation methods including Splat, our own simulation, based on a gamma-Poisson distribution. Splat can simulate single populations of cells, populations with multiple cell types, or differentiation paths.

Citing Articles

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scRDiT: Generating Single-cell RNA-seq Data by Diffusion Transformers and Accelerating Sampling.

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