Proteomic Analysis of Secreted Proteins from Cell Microenvironment

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2017 Sep 2

PMID 28861816

Citations 3

Authors

Subash Adhikari

Lan Chen

Peiwu Huang

Ruijun Tian

Affiliations

Soon will be listed here.

Abstract

Cell microenvironment consists of various types of cells which communicate with each other by vast number of secreted proteins. An unbiased profiling of these secreted proteins on a global scale is often critical for understanding the intercellular signaling in an autocrine or paracrine manner. Mass spectrometry-based proteomics has become one of the most popular technology for characterization of the secreted proteins. In this chapter, we discuss the standard workflow for secreted proteins characterization, including harvesting secreted proteins from conditioned media, digesting the obtained proteins, liquid chromatography-mass spectrometry analysis, and downstream data analysis.

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