Chicken Induced Pluripotent Stem Cells: Establishment and Characterization

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2017 Aug 16

PMID 28809024

Citations 10

Authors

Aurelie Fuet

Bertrand Pain

Affiliations

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Abstract

In mammals, the introduction of the OSKM (Oct4, Sox2, Klf4, and c-Myc) genes into somatic cells has allowed generating induced pluripotent stem (iPS) cells. So far, this process has been only clearly demonstrated in mammals. Here, using chicken as an avian model, we describe a set of protocols allowing the establishment, characterization, maintenance, differentiation, and injection of putative reprogrammed chicken Induced Pluripotent Stem (iPS) cells.

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