A Modified Yeast-one Hybrid System for Heteromeric Protein Complex-DNA Interaction Studies

Overview

Journal J Vis Exp

Specialties Biology
General Medicine

Date 2017 Aug 9

PMID 28784944

Citations 1

Authors

Prateek Tripathi

Jose L Pruneda-Paz

Steve A Kay

Affiliations

Soon will be listed here.

Abstract

Over the years, the yeast one-hybrid assay has proven to be an important technique for the identification and validation of physical interactions between proteins such as transcription factors (TFs) and their DNA target. The method presented here utilizes the underlying concept of the Y1H but is modified further to study and validate protein complexes binding to their target DNA. Hence, it is referred to as the modified yeast one-hybrid (Y1.5H) assay. This assay is cost effective and can be easily performed in a regular laboratory setting. Albeit using a heterologous system, the described method could be a valuable tool to test and validate the heteromeric protein complex binding to their DNA target(s) for functional genomics in any system of study, especially plant genomics.

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