Characteristics of Dihydroflavonol 4-reductase Gene Promoters from Different Leaf Colored Crabapple Cultivars

Overview

Journal Hortic Res

Publisher Oxford University Press

Date 2017 Dec 22

PMID 29263792

Citations 30

Authors

Ji Tian

Meng-Chen Chen

Jie Zhang

Ke-Ting Li

Ting-Ting Song

Xi Zhang

Yun-Cong Yao

Affiliations

Soon will be listed here.

Abstract

Anthocyanins are secondary metabolites in land plants that contribute to the colors of leaves and flowers, and are nutritionally valuable components of the human diet. The gene plays an important role in the anthocyanin biosynthetic pathway. In this study, we investigated the regulation of expression and in different crabapple cultivars that show distinct patterns of leaf coloration, and how it influences leaf anthocyanin accumulation and coloration. Specifically, we studied the ever-red leaved cultivar 'Royalty', the ever-green leaved cultivar 'Flame' and the spring-red leaved cultivar 'Radiant'. RT-PCR analysis showed that the expression of correlated with the expression of a MYB transcription factor, , and with anthocyanin accumulation. We isolated five promoter fragments from the three cultivars and identified four different fragments (F1-4) that were present either in several cultivars, or only in one. Yeast one-hybrid and electrophoretic mobility shift assay analyses showed that McMYB10 could bind to all the promoters, except . The F1, F2 and F3 fragments did not affect McMYB10 binding to the promoters; however, we found evidence that the F4 fragment suppressed binding, and that the MYBGAHV amino-acid sequence maybe an important -element for McMYB10 protein binding. This information has potential value for strategies to modify plant color through genetic transformation.

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