Yeast One-hybrid Assays: a Historical and Technical Perspective

Overview

Journal Methods

Specialty Biochemistry

Date 2012 Aug 14

PMID 22884952

Citations 30

Authors

John S Reece-Hoyes

A J Marian Walhout

Affiliations

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Abstract

Since its development about two decades ago, the yeast one-hybrid (Y1H) assay has become an important technique for detecting physical interactions between sequence-specific regulatory transcription factor proteins (TFs) and their DNA target sites. Multiple versions of the Y1H methodology have been developed, each with technical differences and unique advantages. We will discuss several of these technical variations in detail, and also provide some ideas for how Y1H assays can be further improved.

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