Secondary Metabolites from Endophytic Fungus Chaetomium Sp. Induce Colon Cancer Cell Apoptotic Death

A rare depsipeptide, chaetomiamide A (1), together with two known diketopiperazines (2, 3) were isolated from the cultures of endophytic fungus Chaetomium sp., which was isolated from the root of Cymbidium goeringii. Compound 1 represents a rare skeleton with a 13-membered ring system. It structure was established on the basis of spectroscopic data interpretation. The configuration of 1 was determined by NOESY and Marfey's analysis. These isolates were evaluated for anticancer activity and 3 displayed more potent cytotoxicity than the positive control cisplatin associated with G2/M cell cycle arrest. In addition, 3 induced apoptosis via caspase-3 induction and PARP cleavage, concomitantly with the increase of Bax and decrease of Bcl-2.