Single Molecule Fluorescence in Situ Hybridisation for Quantitating Post-transcriptional Regulation in Drosophila Brains

Overview

Journal Methods

Specialty Biochemistry

Date 2017 Jun 28

PMID 28651965

Citations 23

Authors

Lu Yang

Josh Titlow

Darragh Ennis

Carlas Smith

Jessica Mitchell

Florence L Young

Scott Waddell

David Ish-Horowicz

Ilan Davis

Affiliations

Soon will be listed here.

Abstract

RNA in situ hybridization is a powerful method to investigate post-transcriptional regulation, but analysis of intracellular mRNA distributions in thick, complex tissues like the brain poses significant challenges. Here, we describe the application of single-molecule fluorescent in situ hybridization (smFISH) to quantitate primary nascent transcription and post-transcriptional regulation in whole-mount Drosophila larval and adult brains. Combining immunofluorescence and smFISH probes for different regions of a single gene, i.e., exons, 3'UTR, and introns, we show examples of a gene that is regulated post-transcriptionally and one that is regulated at the level of transcription. Our simple and rapid protocol can be used to co-visualise a variety of different transcripts and proteins in neuronal stem cells as well as deep brain structures such as mushroom body neuropils, using conventional confocal microscopy. Finally, we introduce the use of smFISH as a sensitive alternative to immunofluorescence for labelling specific neural stem cell populations in the brain.

Citing Articles

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