A DNA Origami Platform for Quantifying Protein Copy Number in Super-resolution

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2017 Jun 27

PMID 28650478

Citations 47

Authors

Francesca Cella Zanacchi

Carlo Manzo

Angel S Alvarez

Nathan D Derr

Maria F Garcia-Parajo

Melike Lakadamyali

Affiliations

Soon will be listed here.

Abstract

Single-molecule-based super-resolution microscopy offers researchers a unique opportunity to quantify protein copy number with nanoscale resolution. However, while fluorescent proteins have been characterized for quantitative imaging using calibration standards, similar calibration tools for immunofluorescence with small organic fluorophores are lacking. Here we show that DNA origami, in combination with GFP antibodies, is a versatile platform for calibrating fluorophore and antibody labeling efficiency to quantify protein copy number in cellular contexts using super-resolution microscopy.

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