Pilot Study of Large-scale Production of Mutant Pigs by ENU Mutagenesis

Overview

Journal Elife

Specialty Biology

Date 2017 Jun 23

PMID 28639938

Citations 21

Authors

Tang Hai

Chunwei Cao

Haitao Shang

Weiwei Guo

Yanshuang Mu

Shulin Yang

Ying Zhang

Qiantao Zheng

Tao Zhang

Xianlong Wang

Yu Liu

Qingran Kong

Kui Li

Dayu Wang

Meng Qi

Qianlong Hong

Rui Zhang

Xiupeng Wang

Qitao Jia

Xiao Wang

Guosong Qin

Yongshun Li

Ailing Luo

Weiwu Jin

Jing Yao

Jiaojiao Huang

Hongyong Zhang

Menghua Li

Xiangmo Xie

Xuejuan Zheng

Kenan Guo

Qinghua Wang

Shibin Zhang

Liang Li

Fei Xie

Yu Zhang

Xiaogang Weng

Zhi Yin

Kui Hu

Yimei Cong

Peng Zheng

Hailong Zou

Leilei Xin

Jihan Xia

Jinxue Ruan

Hegang Li

Weiming Zhao

Jing Yuan

Zizhan Liu

Weiwang Gu

Ming Li

Yong Wang

Hongmei Wang

Shiming Yang

Zhonghua Liu

Hong Wei

Jianguo Zhao

Qi Zhou

Anming Meng

Affiliations

Soon will be listed here.

Abstract

N-ethyl-N-nitrosourea (ENU) mutagenesis is a powerful tool to generate mutants on a large scale efficiently, and to discover genes with novel functions at the whole-genome level in flies, zebrafish and mice, but it has never been tried in large model animals. We describe a successful systematic three-generation ENU mutagenesis screening in pigs with the establishment of the Chinese Swine Mutagenesis Consortium. A total of 6,770 G1 and 6,800 G3 pigs were screened, 36 dominant and 91 recessive novel pig families with various phenotypes were established. The causative mutations in 10 mutant families were further mapped. As examples, the mutation of (R109W) in pig causes inner ear malfunctions and mimics human Mondini dysplasia, and upregulated expression of is associated with congenital splay legs. This study demonstrates the feasibility of artificial random mutagenesis in pigs and opens an avenue for generating a reservoir of mutants for agricultural production and biomedical research.

Citing Articles

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Homer 1 genotype AA variant relates to congenital splay leg syndrome in piglets by repressing Pax7 in myogenic progenitors.

Schumacher T, Reyer H, Maak S, Rontgen M Front Vet Sci. 2023; 10:1028879.

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SOX9 and SOX10 control fluid homeostasis in the inner ear for hearing through independent and cooperative mechanisms.

Szeto I, Chu D, Chen P, Chu K, Au T, Leung K Proc Natl Acad Sci U S A. 2022; 119(46):e2122121119.

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