Mutagenesis and Mapping of a Mouse Gene, Clock, Essential for Circadian Behavior

Overview

Journal Science

Specialty Science

Date 1994 Apr 29

PMID 8171325

Citations 506

Authors

M H Vitaterna

D P King

A M Chang

J M Kornhauser

P L Lowrey

J D McDonald

W F Dove

L H Pinto

F W Turek

J S Takahashi

Affiliations

Soon will be listed here.

Abstract

In a search for genes that regulate circadian rhythms in mammals, the progeny of mice treated with N-ethyl-N-nitrosourea (ENU) were screened for circadian clock mutations. A semidominant mutation, Clock, that lengthens circadian period and abolishes persistence of rhythmicity was identified. Clock segregated as a single gene that mapped to the midportion of mouse chromosome 5, a region syntenic to human chromosome 4. The power of ENU mutagenesis combined with the ability to clone murine genes by map position provides a generally applicable approach to study complex behavior in mammals.

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