Speckle-modulating Optical Coherence Tomography in Living Mice and Humans

Overview

Journal Nat Commun

Specialty Biology

Date 2017 Jun 21

PMID 28632205

Citations 43

Authors

Orly Liba

Matthew D Lew

Elliott D SoRelle

Rebecca Dutta

Debasish Sen

Darius M Moshfeghi

Steven Chu

Adam de la Zerda

Affiliations

Soon will be listed here.

Abstract

Optical coherence tomography (OCT) is a powerful biomedical imaging technology that relies on the coherent detection of backscattered light to image tissue morphology in vivo. As a consequence, OCT is susceptible to coherent noise (speckle noise), which imposes significant limitations on its diagnostic capabilities. Here we show speckle-modulating OCT (SM-OCT), a method based purely on light manipulation that virtually eliminates speckle noise originating from a sample. SM-OCT accomplishes this by creating and averaging an unlimited number of scans with uncorrelated speckle patterns without compromising spatial resolution. Using SM-OCT, we reveal small structures in the tissues of living animals, such as the inner stromal structure of a live mouse cornea, the fine structures inside the mouse pinna, and sweat ducts and Meissner's corpuscle in the human fingertip skin-features that are otherwise obscured by speckle noise when using conventional OCT or OCT with current state of the art speckle reduction methods.

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