CRISPR/Cas9 Gene Drives in Genetically Variable and Nonrandomly Mating Wild Populations

Overview

Journal Sci Adv

Specialties Biology
Science

Date 2017 Jun 1

PMID 28560324

Citations 54

Authors

Douglas W Drury

Amy L Dapper

Dylan J Siniard

Gabriel E Zentner

Michael J Wade

Affiliations

Soon will be listed here.

Abstract

Synthetic gene drives based on CRISPR/Cas9 have the potential to control, alter, or suppress populations of crop pests and disease vectors, but it is unclear how they will function in wild populations. Using genetic data from four populations of the flour beetle , we show that most populations harbor genetic variants in Cas9 target sites, some of which would render them immune to drive (ITD). We show that even a rare ITD allele can reduce or eliminate the efficacy of a CRISPR/Cas9-based synthetic gene drive. This effect is equivalent to and accentuated by mild inbreeding, which is a characteristic of many disease-vectoring arthropods. We conclude that designing such drives will require characterization of genetic variability and the mating system within and among targeted populations.

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