Microinfusion of Clonidine and Yohimbine into Locus Coeruleus Alters EEG Power Spectrum: Effects of Aging and Reversal by Phosphatidylserine

Overview

Journal Br J Pharmacol

Publisher Wiley

Specialty Pharmacology

Date 1988 Dec 1

PMID 2851361

Citations 11

Authors

G B De Sarro

G Bagetta

C Ascioti

V Libri

G Nistico

Affiliations

Soon will be listed here.

Abstract

1. The behavioural and electrocortical (ECoG) power spectrum effects of clonidine, and yohimbine, an agonist and an antagonist at alpha 2-adrenoceptors, after their unilateral microinfusion into the rat locus coeruleus (LC) in young (50-70 days old) and old (13-15 months old) rats were studied. 2. Clonidine (0.09, 0.19, 0.28 and 0.56 nmol) microinfused into the LC of young rats induced dose-dependent behavioural and ECoG slow wave sleep (SWS) with a significant increase in total voltage power and power in the lower frequency bands. In contrast, yohimbine (1.3 and 2.6 nmol) infused into the LC of young rats produced ECoG desynchronization and a significant decrease in total voltage power. 3. In contrast to young rats, clonidine (0.19 and 0.28 nmol) given into the LC did not affect behaviour and the ECoG power spectrum in old rats. However, after higher doses of clonidine (0.56 and 1.2 nmol) a small and short-lasting period of behavioural and ECoG SWS was still evident. Similarly, in old rats yohimbine, at a dose (1.3 nmol) which was stimulative in young animals, did not significantly affect behaviour and ECoG power spectrum. Higher doses of yohimbine (2.6 and 5.2 nmol) were required to induce behavioural and ECoG changes similar to those observed with lower doses of yohimbine in young rats. 4. Chronic treatment with phosphatidylserine (30 mg kg-1, orally, daily for 21 and 30 days), was able gradually to restore in old rats, in comparison with a vehicle-treated group, the responsiveness of alpha 2-adrenoceptors to clonidine and yohimbine given into the LC.

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References

Swanson L, Hartman B . The central adrenergic system. An immunofluorescence study of the location of cell bodies and their efferent connections in the rat utilizing dopamine-beta-hydroxylase as a marker. J Comp Neurol. 1975; 163(4):467-505. DOI: 10.1002/cne.901630406. View

Gaiti A, Sitkievicz D, Brunetti M, Porcellati G . Phospholipid metabolism in neuronal and glial cells during aging. Neurochem Res. 1981; 6(1):13-22. DOI: 10.1007/BF00963901. View

Cedarbaum J, Aghajanian G . Catecholamine receptors on locus coeruleus neurons: pharmacological characterization. Eur J Pharmacol. 1977; 44(4):375-85. DOI: 10.1016/0014-2999(77)90312-0. View

Leon A, Benvegnu D, Toffano G, Orlando P, Massari P . Effect of brain cortex phospholipids on adenylate-cyclase activity of mouse brain. J Neurochem. 1978; 30(1):23-6. DOI: 10.1111/j.1471-4159.1978.tb07030.x. View

Amaral D, Sinnamon H . The locus coeruleus: neurobiology of a central noradrenergic nucleus. Prog Neurobiol. 1977; 9(3):147-96. DOI: 10.1016/0301-0082(77)90016-8. View

Bricolo A, Turazzi S, Faccioli F, Odorizzi F, Sciaretta G, Erculiani P . Clinical application of compressed spectral array in long-term EEG monitoring of comatose patients. Electroencephalogr Clin Neurophysiol. 1978; 45(2):211-25. DOI: 10.1016/0013-4694(78)90005-6. View

Casamenti F, Mantovani P, Amaducci L, Pepeu G . Effect of phosphatidylserine on acetylcholine output from the cerebral cortex of the rat. J Neurochem. 1979; 32(2):529-33. DOI: 10.1111/j.1471-4159.1979.tb00380.x. View

Crews F, Calderini G, Battistella A, Toffano G . Age dependent changes in the methylation of rat brain phospholipids. Brain Res. 1981; 229(1):256-9. DOI: 10.1016/0006-8993(81)90767-8. View

Aston-Jones G, Bloom F . Activity of norepinephrine-containing locus coeruleus neurons in behaving rats anticipates fluctuations in the sleep-waking cycle. J Neurosci. 1981; 1(8):876-86. PMC: 6564235. View

10.

Aston-Jones G, Bloom F . Norepinephrine-containing locus coeruleus neurons in behaving rats exhibit pronounced responses to non-noxious environmental stimuli. J Neurosci. 1981; 1(8):887-900. PMC: 6564231. View

11.

Brodde O, Anlauf M, Graben N, Bock K . Age-dependent decrease of alpha 2-adrenergic receptor number in human platelets. Eur J Pharmacol. 1982; 81(2):345-7. DOI: 10.1016/0014-2999(82)90456-3. View

12.

Cespuglio R, Gomez M, Faradji H, Jouvet M . Alterations in the sleep-waking cycle induced by cooling of the locus coeruleus area. Electroencephalogr Clin Neurophysiol. 1982; 54(5):570-8. DOI: 10.1016/0013-4694(82)90042-6. View

13.

Calderini G, Bonetti A, Battistella A, Crews F, Toffano G . Biochemical changes of rat brain membranes with aging. Neurochem Res. 1983; 8(4):483-92. DOI: 10.1007/BF00965104. View

14.

Nagy K, Simon P, Zs-Nagy I . Spin label studies on synaptosomal membranes of rat brain cortex during aging. Biochem Biophys Res Commun. 1983; 117(3):688-94. DOI: 10.1016/0006-291x(83)91652-2. View

15.

Burchinsky S . Neurotransmitter receptors in the central nervous system and aging: pharmacological aspect (review). Exp Gerontol. 1984; 19(4):227-39. DOI: 10.1016/0531-5565(84)90018-4. View

16.

Hamilton C, Howe C, Reid J . Changes in brain alpha-adrenoceptors with increasing age in rabbits. Brain Res. 1984; 322(1):177-9. DOI: 10.1016/0006-8993(84)91201-0. View

17.

Gaiti A, Brunetti M, Gatti C, Porcellati G . Turnover of ethanolamine phosphoglycerides in different brain areas of adult and aged rats. Neurochem Res. 1984; 9(11):1549-58. DOI: 10.1007/BF00964590. View

18.

Schroeder F . Role of membrane lipid asymmetry in aging. Neurobiol Aging. 1984; 5(4):323-33. DOI: 10.1016/0197-4580(84)90010-1. View

19.

Gatti C, Cantelmi M, Brunetti M, Gaiti A, Calderini G, Teolato S . Effect of chronic treatment with phosphatidyl serine on phospholipase A1 and A2 activities in different brain areas of 4 month and 24 month old rats. Farmaco Sci. 1985; 40(7):493-500. View

20.

Bonetti A, Bellini F, Calderini G, Galbiati E, Toffano G . Age-dependent changes in the mechanisms controlling prolactin secretion and phosphatidylinositol turnover in male rats: effect of phosphatidylserine. Neuroendocrinology. 1987; 45(2):123-9. DOI: 10.1159/000124714. View