Dendritic Cells Enter Lymph Vessels by Hyaluronan-mediated Docking to the Endothelial Receptor LYVE-1

Overview

Journal Nat Immunol

Date 2017 May 16

PMID 28504698

Citations 103

Authors

Louise A Johnson

Suneale Banerji

William Lawrance

Uzi Gileadi

Gennaro Prota

Kayla A Holder

Yaowaluck M Roshorm

Tomas Hanke

Vincenzo Cerundolo

Nicholas W Gale

David G Jackson

Affiliations

Soon will be listed here.

Abstract

Trafficking of tissue dendritic cells (DCs) via lymph is critical for the generation of cellular immune responses in draining lymph nodes (LNs). In the current study we found that DCs docked to the basolateral surface of lymphatic vessels and transited to the lumen through hyaluronan-mediated interactions with the lymph-specific endothelial receptor LYVE-1, in dynamic transmigratory-cup-like structures. Furthermore, we show that targeted deletion of the gene Lyve1, antibody blockade or depletion of the DC hyaluronan coat not only delayed lymphatic trafficking of dermal DCs but also blunted their capacity to prime CD8 T cell responses in skin-draining LNs. Our findings uncovered a previously unknown function for LYVE-1 and show that transit through the lymphatic network is initiated by the recognition of leukocyte-derived hyaluronan.

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