5-Formylcytosine Does Not Change the Global Structure of DNA

Overview

Journal Nat Struct Mol Biol

Specialties Cell Biology
Molecular Biology

Date 2017 May 16

PMID 28504696

Citations 21

Authors

Jack S Hardwick

Denis Ptchelkine

Afaf H El-Sagheer

Ian Tear

Daniel Singleton

Simon E V Phillips

Andrew N Lane

Tom Brown

Affiliations

Soon will be listed here.

Abstract

The mechanism by which the recently identified DNA modification 5-formylcytosine (C) is recognized by epigenetic writer and reader proteins is not known. Recently, an unusual DNA structure, F-DNA, has been proposed as the basis for enzyme recognition of clusters of C. We used NMR and X-ray crystallography to compare several modified DNA duplexes with unmodified analogs and found that in the crystal state the duplexes all belong to the A family, whereas in solution they are all members of the B family. We found that, contrary to previous findings, C does not significantly affect the structure of DNA, although there are modest local differences at the modification sites. Hence, global conformation changes are unlikely to account for the recognition of this modified base, and our structural data favor a mechanism that operates at base-pair resolution for the recognition of C by epigenome-modifying enzymes.

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