Locus-specific Histone Deacetylation Using a Synthetic CRISPR-Cas9-based HDAC

Overview

Journal Nat Commun

Specialty Biology

Date 2017 May 13

PMID 28497787

Citations 112

Authors

Deborah Y Kwon

Ying-Tao Zhao

Janine M Lamonica

Zhaolan Zhou

Affiliations

Soon will be listed here.

Abstract

Efforts to manipulate locus-specific histone acetylation to assess their causal role in gene expression and cellular and behavioural phenotypes have been impeded by a lack of experimental tools. The Cas9 nuclease has been adapted to target epigenomic modifications, but a detailed description of the parameters of such synthetic epigenome remodellers is still lacking. Here we describe a Cas9-based histone deacetylase (HDAC) and the design principles required to achieve locus-specific histone deacetylation. We assess its range of activity and specificity, and analyse target gene expression in two different cell types to investigate cellular context-dependent effects. Our findings demonstrate that the chromatin environment is an important element to consider when utilizing this synthetic HDAC.

Citing Articles

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