Sequence Imperfections and Base Triplet Recognition by the Rad51/RecA Family of Recombinases

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 2017 May 7

PMID 28476890

Citations 17

Authors

Ja Yil Lee

Justin B Steinfeld

Zhi Qi

Youngho Kwon

Patrick Sung

Eric C Greene

Affiliations

Soon will be listed here.

Abstract

Homologous recombination plays key roles in double-strand break repair, rescue, and repair of stalled replication forks and meiosis. The broadly conserved Rad51/RecA family of recombinases catalyzes the DNA strand invasion reaction that takes place during homologous recombination. We have established single-stranded (ss)DNA curtain assays for measuring individual base triplet steps during the early stages of strand invasion. Here, we examined how base triplet stepping by RecA, Rad51, and Dmc1 is affected by DNA sequence imperfections, such as single and multiple mismatches, abasic sites, and single nucleotide insertions. Our work reveals features of base triplet stepping that are conserved among these three phylogenetic lineages of the Rad51/RecA family and also reveals lineage-specific behaviors reflecting properties that are unique to each recombinase. These findings suggest that Dmc1 is tolerant of single mismatches, multiple mismatches, and even abasic sites, whereas RecA and Rad51 are not. Interestingly, the presence of single nucleotide insertion abolishes recognition of an adjacent base triplet by all three recombinases. On the basis of these findings, we describe models for how sequence imperfections may affect base triplet recognition by Rad51/RecA family members, and we discuss how these models and our results may relate to the different biological roles of RecA, Rad51, and Dmc1.

Citing Articles

The kinase ATR controls meiotic crossover distribution at the genome scale in Arabidopsis.

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Hop2-Mnd1 functions as a DNA sequence fidelity switch in Dmc1-mediated DNA recombination.

Peng J, Chang H, Sun Y, Prentiss M, Li H, Chi P Nat Commun. 2024; 15(1):9266.

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Recombinase-independent chromosomal rearrangements between dispersed inverted repeats in Saccharomyces cerevisiae meiosis.

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Repair of mismatched templates during Rad51-dependent Break-Induced Replication.

Choi J, Kong M, Gallagher D, Li K, Bronk G, Cao Y PLoS Genet. 2022; 18(9):e1010056.

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DMC1 attenuates RAD51-mediated recombination in Arabidopsis.

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