Degradation of SERRATE Via Ubiquitin-independent 20S Proteasome to Survey RNA Metabolism

Overview

Journal Nat Plants

Specialties Biology
Genetics

Date 2020 Jul 22

PMID 32690892

Citations 25

Authors

Yanjun Li

Di Sun

Zeyang Ma

Karissa Yamaguchi

Lin Wang

Songxiao Zhong

Xingxing Yan

Baoshuan Shang

Yukihiro Nagashima

Hisashi Koiwa

Jiajia Han

Qi Xie

Mingguo Zhou

Zhiye Wang

Xiuren Zhang

Affiliations

Soon will be listed here.

Abstract

SERRATE (SE) is a key factor in RNA metabolism. Here, we report that SE binds 20S core proteasome α subunit G1 (PAG1) among other components and is accumulated in their mutants. Purified PAG1-containing 20S proteasome degrades recombinant SE via an ATP- and ubiquitin-independent manner in vitro. Nevertheless, PAG1 is a positive regulator for SE in vivo, as pag1 shows comparable molecular and/or developmental defects relative to se. Furthermore, SE is poorly assembled into macromolecular complexes, exemplified by the microprocessor in pag1 compared with Col-0. SE overexpression triggered the destruction of both transgenic and endogenous protein, leading to similar phenotypes of se and SE overexpression lines. We therefore propose that PAG1 degrades the intrinsically disordered portion of SE to secure the functionality of folded SE that is assembled and protected in macromolecular complexes. This study provides insight into how the 20S proteasome regulates RNA metabolism through controlling its key factor in eukaryotes.

Citing Articles

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