Phosphorylation of Serum Response Factor, a Factor That Binds to the Serum Response Element of the C-FOS Enhancer

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1988 Oct 1

PMID 2845402

Citations 64

Authors

R Prywes

A Dutta

J A Cromlish

R G Roeder

Affiliations

Soon will be listed here.

Abstract

Serum and growth factor regulation of c-FOS protooncogene transcription is mediated by the serum response element. A factor, serum response factor, binding to this element has already been identified. We demonstrate that serum response factor is phosphorylated in vivo on serine residues and that phosphatase treatment of this factor in vitro abolishes its DNA-binding activity. These results show phosphorylation of serum response factor to be required for its DNA-binding activity. The importance of serum response factor phosphorylation for the regulation of c-FOS expression is discussed.

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