The Centrosomal OFD1 Protein Interacts with the Translation Machinery and Regulates the Synthesis of Specific Targets

Overview

Journal Sci Rep

Specialty Science

Date 2017 Apr 29

PMID 28450740

Citations 22

Authors

Daniela Iaconis

Maria Monti

Mario Renda

Arianne van Koppen

Roberta Tammaro

Marco Chiaravalli

Flora Cozzolino

Paola Pignata

Claudia Crina

Piero Pucci

Alessandra Boletta

Vincenzo Belcastro

Rachel H Giles

Enrico Maria Surace

Simone Gallo

Mario Pende

Brunella Franco

Affiliations

Soon will be listed here.

Abstract

Protein synthesis is traditionally associated with specific cytoplasmic compartments. We now show that OFD1, a centrosomal/basal body protein, interacts with components of the Preinitiation complex of translation (PIC) and of the eukaryotic Initiation Factor (eIF)4F complex and modulates the translation of specific mRNA targets in the kidney. We demonstrate that OFD1 cooperates with the mRNA binding protein Bicc1 to functionally control the protein synthesis machinery at the centrosome where also the PIC and eIF4F components were shown to localize in mammalian cells. Interestingly, Ofd1 and Bicc1 are both involved in renal cystogenesis and selected targets were shown to accumulate in two models of inherited renal cystic disease. Our results suggest a possible role for the centrosome as a specialized station to modulate translation for specific functions of the nearby ciliary structures and may provide functional clues for the understanding of renal cystic disease.

Citing Articles

Conservation of OFD1 Protein Motifs: Implications for Discovery of Novel Interactors and the OFD1 Function.

Jagodzik P, Zietkiewicz E, Bukowy-Bieryllo Z Int J Mol Sci. 2025; 26(3).

PMID: 39940934 PMC: 11818881. DOI: 10.3390/ijms26031167.

Radial glia progenitor polarity in health and disease.

Viola V, Chinnappa K, Francis F Front Cell Dev Biol. 2024; 12:1478283.

PMID: 39416687 PMC: 11479994. DOI: 10.3389/fcell.2024.1478283.

Antagonistic interactions among structured domains in the multivalent Bicc1-ANKS3-ANKS6 protein network govern phase transitioning of target mRNAs.

Rothe B, Fortier S, Gagnieux C, Schmuziger C, Constam D iScience. 2023; 26(6):106855.

PMID: 37275520 PMC: 10232731. DOI: 10.1016/j.isci.2023.106855.

Single-cell proteo-genomic reveals a comprehensive map of centrosome-associated spliceosome components.

Cerulo L, Pezzella N, Caruso F, Parente P, Remo A, Giordano G iScience. 2023; 26(5):106602.

PMID: 37250316 PMC: 10214398. DOI: 10.1016/j.isci.2023.106602.

A local translation program regulates centriole amplification in the airway epithelium.

Liu H, Li H, Jiang Z, Jin S, Song R, Yang Y Sci Rep. 2023; 13(1):7090.

PMID: 37127654 PMC: 10151349. DOI: 10.1038/s41598-023-34365-8.

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