Particle Agglutination Assays for Rapid Detection of Fibronectin, Fibrinogen, and Collagen Receptors on Staphylococcus Aureus

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1988 Aug 1

PMID 2844847

Citations 13

Authors

A S Naidu

M Paulsson

T Wadstrom

Affiliations

Soon will be listed here.

Abstract

Latex beads (0.8-micron diameter; Difco Laboratories) were coated with fibronectin, fibrinogen, collagen type I, or denatured collagen (gelatin) and evaluated in a particle agglutination assay (PAA) for the rapid detection of fibronectin, fibrinogen, or collagen binding to Staphylococcus aureus. These assays were compared with a commercial test for detecting the binding of fibrinogen and immunoglobulin G (Staphaurex). Bacterial cells (approximately 10(10) cells per ml) suspended in 0.02 M potassium phosphate buffer (pH 6.8) caused the clumping of standard fibronectin, collagen, gelatin, and fibrinogen latex suspensions within 2 min on glass slides. The test results were scored semiquantitatively from strongly positive ( ) to weakly positive (+) and negative (-) reactions. The negative PAA reactions corresponded to a median value of 11.5% relative to the binding of 125I-labeled protein to strain Cowan 1, indicating the high sensitivity of the test. The reactions with fibronectin and fibrinogen latex suspensions and with Staphaurex were optimal for cells grown on tryptic soy and brain heart infusion broth media. Blood agar was optimal for reactions with collagen and gelatin latex suspensions. Media containing high salts (mannitol salt agar and staphylococcus medium 110) enhanced the tendency of cells to autoaggregate. These assays were also clinically evaluated on 187 S. aureus isolates. The PAA reagents were stable, and the assays were highly specific, sensitive, and reproducible, thus making PAA suitable for the rapid screening of the binding of various bacterial pathogens to serum and connective-tissue proteins.

Citing Articles

Candida albicans-epithelial interactions: dissecting the roles of active penetration, induced endocytosis and host factors on the infection process.

Wachtler B, Citiulo F, Jablonowski N, Forster S, Dalle F, Schaller M PLoS One. 2012; 7(5):e36952.

PMID: 22606314 PMC: 3351431. DOI: 10.1371/journal.pone.0036952.

Mode of binding of fibrinogen, fibronectin and iron-binding proteins by animal enterococci.

Styriak I, Laukova A, Strompfova V, Ljungh A Vet Res Commun. 2004; 28(7):587-98.

PMID: 15563106 DOI: 10.1023/b:verc.0000042865.63246.de.

rgf encodes a novel two-component signal transduction system of Streptococcus agalactiae.

Spellerberg B, Rozdzinski E, Martin S, Weber-Heynemann J, Lutticken R Infect Immun. 2002; 70(5):2434-40.

PMID: 11953380 PMC: 127907. DOI: 10.1128/IAI.70.5.2434-2440.2002.

Use of commercial particle agglutination systems for the rapid identification of methicillin-susceptible and methicillin-resistant Staphylococcus aureus.

Adams J, Van Enk R Eur J Clin Microbiol Infect Dis. 1994; 13(1):86-9.

PMID: 8168569 DOI: 10.1007/BF02026132.

Cell surface hydrophobicity and adherence to extra-cellular matrix proteins in two collections of methicillin-resistant Staphylococcus aureus.

Cree R, Aleljung P, Paulsson M, Witte W, Noble W, Ljungh A Epidemiol Infect. 1994; 112(2):307-14.

PMID: 8150005 PMC: 2271452. DOI: 10.1017/s0950268800057721.

References

Vuento M, Vaheri A . Purification of fibronectin from human plasma by affinity chromatography under non-denaturing conditions. Biochem J. 1979; 183(2):331-7. PMC: 1161563. DOI: 10.1042/bj1830331. View

Shands K, Schmid G, Dan B, Blum D, Guidotti R, Hargrett N . Toxic-shock syndrome in menstruating women: association with tampon use and Staphylococcus aureus and clinical features in 52 cases. N Engl J Med. 1980; 303(25):1436-42. DOI: 10.1056/NEJM198012183032502. View

Beachey E . Bacterial adherence: adhesin-receptor interactions mediating the attachment of bacteria to mucosal surface. J Infect Dis. 1981; 143(3):325-45. DOI: 10.1093/infdis/143.3.325. View

Essers L, Radebold K . Rapid and reliable identification of Staphylococcus aureus by a latex agglutination test. J Clin Microbiol. 1980; 12(5):641-3. PMC: 273662. DOI: 10.1128/jcm.12.5.641-643.1980. View

Miller E, RHODES R . Preparation and characterization of the different types of collagen. Methods Enzymol. 1982; 82 Pt A:33-64. DOI: 10.1016/0076-6879(82)82059-4. View

Ruoslahti E, Hayman E, Pierschbacher M, Engvall E . Fibronectin: purification, immunochemical properties, and biological activities. Methods Enzymol. 1982; 82 Pt A:803-31. DOI: 10.1016/0076-6879(82)82103-4. View

Espersen F, Clemmensen I . Isolation of a fibronectin-binding protein from Staphylococcus aureus. Infect Immun. 1982; 37(2):526-31. PMC: 347566. DOI: 10.1128/iai.37.2.526-531.1982. View

Speziale P, Hook M, Wadstrom T, Timpl R . Binding of the basement membrane protein laminin to Escherichia coli. FEBS Lett. 1982; 146(1):55-8. DOI: 10.1016/0014-5793(82)80704-7. View

Carret G, Emonard H, Fardel G, Druguet M, Herbage D, Flandrois J . Gelatin and collagen binding to Staphylococcus aureus strains. Ann Inst Pasteur Microbiol (1985). 1985; 136A(2):241-5. DOI: 10.1016/s0769-2609(85)80063-6. View

10.

Lopes J, dos Reis M, Brentani R . Presence of laminin receptors in Staphylococcus aureus. Science. 1985; 229(4710):275-7. DOI: 10.1126/science.3160113. View

11.

Vercellotti G, McCarthy J, Lindholm P, Peterson P, JACOB H, Furcht L . Extracellular matrix proteins (fibronectin, laminin, and type IV collagen) bind and aggregate bacteria. Am J Pathol. 1985; 120(1):13-21. PMC: 1887975. View

12.

Kuusela P, Vartio T, Vuento M, Myhre E . Attachment of staphylococci and streptococci on fibronectin, fibronectin fragments, and fibrinogen bound to a solid phase. Infect Immun. 1985; 50(1):77-81. PMC: 262138. DOI: 10.1128/iai.50.1.77-81.1985. View

13.

Fuquay J, Loo D, Barnes D . Binding of Staphylococcus aureus by human serum spreading factor in an in vitro assay. Infect Immun. 1986; 52(3):714-7. PMC: 260916. DOI: 10.1128/iai.52.3.714-717.1986. View

14.

Thomas D, Baseman J, Alderete J . Enhanced levels of attachment of fibronectin-primed Treponema pallidum to extracellular matrix. Infect Immun. 1986; 52(3):736-41. PMC: 260920. DOI: 10.1128/iai.52.3.736-741.1986. View

15.

Maxe I, Ryden C, Wadstrom T, Rubin K . Specific attachment of Staphylococcus aureus to immobilized fibronectin. Infect Immun. 1986; 54(3):695-704. PMC: 260225. DOI: 10.1128/iai.54.3.695-704.1986. View

16.

Flock J, Froman G, Jonsson K, Guss B, Signas C, Nilsson B . Cloning and expression of the gene for a fibronectin-binding protein from Staphylococcus aureus. EMBO J. 1987; 6(8):2351-7. PMC: 553639. DOI: 10.1002/j.1460-2075.1987.tb02511.x. View

17.

Wadstrom T . Molecular aspects on pathogenesis of wound and foreign body infections due to staphylococci. Zentralbl Bakteriol Mikrobiol Hyg A. 1987; 266(1-2):191-211. DOI: 10.1016/s0176-6724(87)80032-9. View

18.

Mamo W, Froman G, Sundas A, Wadstrom T . Binding of fibronectin, fibrinogen and type II collagen to streptococci isolated from bovine mastitis. Microb Pathog. 1987; 2(6):417-24. DOI: 10.1016/0882-4010(87)90048-9. View

19.

GREENWOOD F, Hunter W, GLOVER J . THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963; 89:114-23. PMC: 1202279. DOI: 10.1042/bj0890114. View

20.

Simpson W, Beachey E . Adherence of group A streptococci to fibronectin on oral epithelial cells. Infect Immun. 1983; 39(1):275-9. PMC: 347937. DOI: 10.1128/iai.39.1.275-279.1983. View