Particle Agglutination Assays to Identify Fibronectin and Collagen Cell Surface Receptors and Lectins in Aeromonas and Vibrio Species

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1990 Jun 1

PMID 2166473

Citations 7

Authors

F Ascencio

P Aleljung

T Wadstrom

Affiliations

Soon will be listed here.

Abstract

A rapid particle agglutination assay (PAA) utilizing latex beads coated with connective tissue and serum proteins was evaluated for its ability to identify fibronectin, collagen (types I and IV), fibrinogen, and transferrin cell surface receptors on Vibrio and Aeromonas strains isolated from diseased fish, human infections, and the environment. Similar tests were performed to screen for cell surface lectins. Vibrio as well as Aeromonas strains were found to bind connective tissue proteins (collagen types I, II, and IV and fibronectin), serum proteins (i.e., fibrinogen), and glycoproteins (bovine submaxillary mucin, hog gastric mucin, orosomucoid, and fetuin) immobilized on the latex particles. The specificity of the agglutination reaction was studied by particle agglutination inhibition assays performed by preincubating bacterial suspensions in solutions containing either gelatin (for the various connective tissue protein PAA reagents) or sialic acid-rich glycoproteins (for the various glycoprotein PAA reagents). Expression of cell surface receptors for connective tissue proteins was found to depend on culture methods.

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References

Miller C, PATTERSON W, JOHNSON P, Swartzell C, Wogoman F, Albarella J . Detection of bacteria by hybridization of rRNA with DNA-latex and immunodetection of hybrids. J Clin Microbiol. 1988; 26(7):1271-6. PMC: 266591. DOI: 10.1128/jcm.26.7.1271-1276.1988. View

. Research on Aeromonas and Plesiomonas. IV. Virulence factors. Experientia. 1987; 43(4):367-74. View

Santos Y, Toranzo A, Barja J, Nieto T, Villa T . Virulence properties and enterotoxin production of Aeromonas strains isolated from fish. Infect Immun. 1988; 56(12):3285-93. PMC: 259736. DOI: 10.1128/iai.56.12.3285-3293.1988. View

Kallenius G, Mollby R, Svenson S, Helin I, Hultberg H, Cedergren B . Occurrence of P-fimbriated Escherichia coli in urinary tract infections. Lancet. 1981; 2(8260-61):1369-72. DOI: 10.1016/s0140-6736(81)92797-5. View

Froman G, Switalski L, Faris A, Wadstrom T, Hook M . Binding of Escherichia coli to fibronectin. A mechanism of tissue adherence. J Biol Chem. 1984; 259(23):14899-905. View

Kabir S, Ali S . Characterization of surface properties of Vibrio cholerae. Infect Immun. 1983; 39(3):1048-58. PMC: 348062. DOI: 10.1128/iai.39.3.1048-1058.1983. View

de Man P, Cedergren B, Enerback S, Larsson A, Leffler H, Lundell A . Receptor-specific agglutination tests for detection of bacteria that bind globoseries glycolipids. J Clin Microbiol. 1987; 25(2):401-6. PMC: 265908. DOI: 10.1128/jcm.25.2.401-406.1987. View

Isaacs R, Paviour S, Bunker D, Lang S . Wound infection with aerogenic Aeromonas strains: a review of twenty-seven cases. Eur J Clin Microbiol Infect Dis. 1988; 7(3):355-60. DOI: 10.1007/BF01962336. View

Atkinson H, Trust T . Hemagglutination properties and adherence ability of Aeromonas hydrophila. Infect Immun. 1980; 27(3):938-46. PMC: 550865. DOI: 10.1128/iai.27.3.938-946.1980. View

10.

Ljungh A, Osterlind M, Wadstrom T . Cell surface hydrophobicity of group D and viridans streptococci isolated from patients with septicaemia. Zentralbl Bakteriol Mikrobiol Hyg A. 1986; 261(3):280-6. DOI: 10.1016/s0176-6724(86)80045-1. View

11.

Johansson G . Partition of proteins and micro-organisms in aqueous biphasic systems. Mol Cell Biochem. 1974; 4(3):169-80. DOI: 10.1007/BF01731478. View

12.

Markwell M . A new solid-state reagent to iodinate proteins. I. Conditions for the efficient labeling of antiserum. Anal Biochem. 1982; 125(2):427-32. DOI: 10.1016/0003-2697(82)90025-2. View

13.

Larsen J, Mellergaard S . Agglutination Typing of Vibrio anguillarum Isolates from Diseased Fish and from the Environment. Appl Environ Microbiol. 1984; 47(6):1261-5. PMC: 240214. DOI: 10.1128/aem.47.6.1261-1265.1984. View

14.

Paula S, Duffey P, Abbott S, Kokka R, Oshiro L, Janda J . Surface properties of autoagglutinating mesophilic aeromonads. Infect Immun. 1988; 56(10):2658-65. PMC: 259626. DOI: 10.1128/iai.56.10.2658-2665.1988. View

15.

Yamamoto T, Kamano T, Uchimura M, Iwanaga M, Yokota T . Vibrio cholerae O1 adherence to villi and lymphoid follicle epithelium: in vitro model using formalin-treated human small intestine and correlation between adherence and cell-associated hemagglutinin levels. Infect Immun. 1988; 56(12):3241-50. PMC: 259731. DOI: 10.1128/iai.56.12.3241-3250.1988. View

16.

Naidu A, Paulsson M, Wadstrom T . Particle agglutination assays for rapid detection of fibronectin, fibrinogen, and collagen receptors on Staphylococcus aureus. J Clin Microbiol. 1988; 26(8):1549-54. PMC: 266658. DOI: 10.1128/jcm.26.8.1549-1554.1988. View

17.

Lammler C, Frede C, Gurturk K, Hildebrand A, Blobel H . Binding activity of Streptococcus canis for albumin and other plasma proteins. J Gen Microbiol. 1988; 134(8):2317-23. DOI: 10.1099/00221287-134-8-2317. View

18.

Trust T, Courtice I, Khouri A, Crosa J, Schiewe M . Serum resistance and hemagglutination ability of marine vibrios pathogenic for fish. Infect Immun. 1981; 34(3):702-7. PMC: 350927. DOI: 10.1128/iai.34.3.702-707.1981. View

19.

Essers L, Radebold K . Rapid and reliable identification of Staphylococcus aureus by a latex agglutination test. J Clin Microbiol. 1980; 12(5):641-3. PMC: 273662. DOI: 10.1128/jcm.12.5.641-643.1980. View

20.

Gonzalez E, Baloda S, Blanco J, Wadstrom T . Growth conditions for the expression of fibronectin and collagen binding to Salmonella. Zentralbl Bakteriol Mikrobiol Hyg A. 1988; 269(4):437-46. DOI: 10.1016/s0176-6724(88)80065-8. View